troubleshooting Protocols

Category: Chromatography Protocols: Column Chromatography Protocols

Column Chromatography Protocol DOC, includes troubleshooting information. Equipment used included: Peristaltic pump, UV detector , Chart recorder, and Column. Prestegard Lab. - [Read Column Chromatography Protocol DOC]

Category: Microscopy Protocols: Confocal Microscopy Protocols

Basic information on confocal microscopy, includes: Specimen Preparation and Imaging; Objective Lens Parameters and Optical Section Thickness; The Objective Lens; Probes for Confocal Imaging; Autofluorescence; Collecting Images; Troubleshooting; Image Processing and Publication; - [Read Confocal Microscopy: Speciman Preparation and Imaging]

Category: Nucleic Acid Modification Protocols: DNA Methylation Protocols

Protocol for DNA-methyltransferase assay. Includes: Homogenize Cells/Tissues; Quantitate Protein Concentration; Dilute Samples; The Assay Proper; Troubleshooting. - [Read DNA-Methyltransferase Assay Protocol]

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: Troubleshooting Guides and FAQs - Stem Cells and ES Cells

ES Cell - Stem Cell Troubleshooting Guide. ESGRO - [Read ES Cell - Stem Cell Troubleshooting Guide - ESGRO]

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

FISH Guide and Troubleshooting. Includes: Cytogenetic techniques; Slide preparation; When do chromosomes spread; Cell suspensions; G-banding; Cell fixatives; Slide preparation factors. - [Read FISH Guide and Troubleshooting]

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

hES - Human Embryonic Stem Cell Culture Manual. Excellent Manual with Pictures. Troubleshooting guide included. Many Topics Included. ES Cell International Pte Ltd. - [Read Human Embryonic Stem Cell Culture Manual]

Category: Protein Protocols: Immunoprecipitation Protocols: Immunoprecipitation Troubleshooting

Immunoprecipitation Troubleshooting Guide. Stressgen. Problem: Non-specific background, Specific Background, Specific antigen not detected. With Solutions to common IP problems. - [Read Immunoprecipitation Troubleshooting Guide PDF]

Category: Protein Protocols: Immunoprecipitation Protocols: Immunoprecipitation Troubleshooting

Immunoprecipitation TroubleShooting Guide. Sigma Aldrich. Problems: No binding, High background or unwanted proteins precipitate. With Solutions. - [Read Immunoprecipitation TroubleShooting Guide Sigma]

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription. Includes information on: Hot transcription reactions, notes and troubleshooting. - [Read In Vitro Transcription Protocol]

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. A variety of downstream applications exist: DNA genotyping and loss-of-heterozygosity (LOH) analysis, etc. Protocol provides a thorough description of LCM techniques, with an emphasis on tips and troubleshooting advice derived from LCM users. The total time required to carry out this protocol is typically 1–1.5 h. - [Read Laser-capture Microdissection Protocol]

Category: PCR Protocols: Long PCR Protocol. Extended PCR.

Long Template PCR. Troubleshooting Long PCR, Amplification of Long PCR Fragments, Roche Guide. - [Read Long Template PCR. Roche Guide.]

Category: DNA Microarray Protocols: Troubleshooting DNA Microarray

Images present some common problems encountered during microarray production, with possible solutions. Galbraith laboratory. - [Read Microarray troubleshooting: image gallery]

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The MTT Cell Proliferation Assay measures the cell proliferation rate and conversely, when metabolic events lead to apoptosis or necrosis, the reduction in cell viability. The number of assay steps has been minimized as much as possible to expedite sample processing. The MTT Reagent yields low background absorbance values in the absence of cells. Includes: Determining optimal cell counts, performing an assay, data interpretations and troubleshooting. - [Read MTT Cell Proliferation Assay Protocol]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

PCR Tips and Tricks - General Guidelines and Troubleshooting PCR polymerase chain reaction. The Genomic Variation Laboratory, U.C Davis - [Read PCR Tips and Tricks]

Category: PCR Protocols: PCR Optimization

PCR Tips and Tricks - General Guidelines and Troubleshooting PCR polymerase chain reaction. The Genomic Variation Laboratory, U.C Davis - [Read PCR Tips and Tricks]

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Restriction Enzyme Troubleshooting Guide. Fermentas. Problems: Undigested or incompletely digested DNA, additional bands atypical, Diffused DNA zones, Low fragment ligation efficiency. - [Read Restriction Enzyme Troubleshooting Guide]

Category: ELISA Protocols: Sandwich ELISA Assay Protocols

Sandwich ELISA Protocol. Chemicon. General Protocol for the Sandwich ELISA method, Troubleshooting Sandiwich ELISA Assays. - [Read Sandwich ELISA Protocol]

Category: Protein Protocols: Immunoprecipitation Protocols: Immunoprecipitation Troubleshooting

Troubleshoot Immunoprecipitation. Chemicon. The most common challenge with immunoprecipitation is trying to lower the number and type of background proteins that contaminate the washed immune complexes. Suggestions for decreasing background in IP. - [Read Troubleshoot Immunoprecipitation]

Category: DNA Microarray Protocols: Troubleshooting DNA Microarray

Troubleshooting Arabidopsis Oligonucleotide Microarrays, David Galbraith. - [Read Troubleshooting Arabidopsis Oligonucleotide Microarrays]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Troubleshooting

Very Good Troubleshooting for PCR and multiplex PCR Guide. Octavian Henegariu. - [Read Troubleshooting for PCR and multiplex PCR]

Category: PCR Protocols: PCR Troubleshooting

Very Good Troubleshooting for PCR and multiplex PCR Guide. Octavian Henegariu. - [Read Troubleshooting for PCR and multiplex PCR]

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Troubleshooting Guide for Chemiluminescent Western Blotting Pierce. High Background, Reverse image on film, Blot glows in the darkroom, Signal duration is less than 8 hours, Weak or no signal, Spots within the protein bands, non-specific bands. - [Read Troubleshooting Guide for Chemiluminescent Western Blotting Pierce]

Category: DNA Microarray Protocols: Troubleshooting DNA Microarray

Troubleshooting Guide to Microarray Printing. Arrayit. - [Read Troubleshooting Guide to Microarray Printing]

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Problem: Poor Transfer, Problem: Poor Transfer. R&D Systems. - [Read Troubleshooting Guide: Western Blot]

Category: Protein Protocols: Immunoprecipitation Protocols

Troubleshooting Immunoprecipitation. Roche. - [Read Troubleshooting Immunoprecipitation]