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Bradford Assay Method - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Biochemistry/Bradford.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue to protein. There is no interference from cations nor from carbohydrates such as sucrose.
Detergents such as sodium dodecyl sulfate and triton x-100 can interfere with the assay, as well as strongly alkaline solutions.

Includes a general overview of the procedure and preparation of the standards in the protocol. - [Read Bradford Assay Method]

Immunohistochemistry Milk Method with 0.01% Triton X-100 Protocol - http://www.bcm.edu/rosenlab/protocols/milkmethod.pdf

Category: Immunohistochemistry Protocols

Immunohistochemistry using the “milk method” with 0.01% triton X-100. This method works for the following antibodies: p27, cyclin D1, RbAP46. - [Read Immunohistochemistry Milk Method with 0.01% Triton X-100 Protocol]

Preparation of Plasmid DNA by Large-scale Boiling Lysis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3910

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Plasmid DNA is isolated from large-scale (500 ml) bacterial cultures by treatment with Triton X-100 and lysozyme, followed by heating. This method is not recommended for preparing plasmid DNA from strains of E. coli that express endonuclease A (endA+ strains). - [Read Preparation of Plasmid DNA by Large-scale Boiling Lysis Protocol]

Preparation of Plasmid DNA by Small-scale Boiling Lysis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3903

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Plasmid DNA is isolated from small-scale (1-2 ml) bacterial cultures by treatment with Triton X-100 and lysozyme, followed by heating. This method is not recommended for preparing plasmid DNA from strains of E. coli that express endonuclease A (endA+ strains). - [Read Preparation of Plasmid DNA by Small-scale Boiling Lysis Protocol]

Protocol for JNK Kinase Assay - http://www.whitelabs.org/Lab%20Protocols/kinase%20and%20phosphatase%20assays/JNK%20Kinase%20Assay.htm

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describing a JNK kinase asssay. Protocol includes information on: Triton Lysis Buffer, Kinase Assay Buffer, Kinase Assay and Preparation of kinase - [Read Protocol for JNK Kinase Assay]

S20 Purification of detergent-insoluble lipid rafts from cells and tissues. - http://www.axis-shield.com/densityhome/optiprep/S20.pdf

Category: Cell Biology and Cell Culture

Protocol for the isolation of the lipid-rich microdomains of the plasma membrane, notably caveolae and lipid rafts. Methods for the isolation of lipid rafts are based on the insolubility of these structures in the nonionic detergent TritonX-100. Either the intact cells are treated with a detergent-containing solution or a post-nuclear supernatant is prepared from a cell homogenate and then Triton X-100 is added to this supernatant. - [Read S20 Purification of detergent-insoluble lipid rafts from cells and tissues.]

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In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.