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Immunoprecipitation: Denaturing Lysis Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4534

Category: Immunology: Immunoprecipitation Protocols

For many sources of antigens, one useful method of lysis is to treat cells with harsh, denaturing solutions to release most of the protein antigens, as described here. The lysates are then diluted to reduce the denaturing conditions to levels that are suitable for the formation of antibody-antigen complexes. The resulting solution is precleared prior to immunoprecipitation. - [Read Immunoprecipitation: Denaturing Lysis Protocol]

Kill Bed Bugs - http://www.killbedbugs.org

Category: Affiliates

Kill Bed Bugs. Information on how to treat, control and manage bedbug infestations. - [Read Kill Bed Bugs]

Phosphatase Treatment of Linearized Vector Protocol - http://openwetware.org/wiki/Phosphatase_treatment_of_linearized_vector

Category: Cloning Protocols: Vector Protocols

To minimize self-ligated vector in your transformation, treat your linearized vector with a phosphatase to remove the 5' phosphates necessary for ligation. This should improve the percentage of colonies with inserts. - [Read Phosphatase Treatment of Linearized Vector Protocol]

Singel Nucleotide Primer Extension (SNuPE) - http://www.methods.info/Methods/DNA_methylation/SNuPE.html

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Single Nucleotide Primer Extension can be used for the analysis of methylation in a certain position. Treat DNA with bisulphite and then anneal a primer which ends immediately before the site of analysis. Dr. A. Gratchev Methods.info - [Read Singel Nucleotide Primer Extension (SNuPE)]

Treat MOUSE EMBRYONIC FIBROBLASTS (MEFs) with Mitomycin C - http://www.uphs.upenn.edu/abramson/simonPlasmids.html

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: MEF Protocols Mouse Embryonic Fibroblasts

Treat MOUSE EMBRYONIC FIBROBLASTS (MEFs) with Mitomycin C - [Read Treat MOUSE EMBRYONIC FIBROBLASTS (MEFs) with Mitomycin C]

Articles (1-5 of 5)

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

Thionin Staining

Thionin Staining method.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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