transport Protocols

Category: Animal Research Techniques

Guinea Pig Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Guinea Pig]

Category: Animal Research Techniques

Mus mouse Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Mouse]

Category: Animal Research Techniques

Rabbit Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Rabbit]

Category: Animal Research Techniques

Rat Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Rat]

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

Accumulation of lipophilic substances in the plasma membrane may affect the membrane lipid order and consequently affect the function of these proteins.  Changes in the activity of the Na+/K+ -ATPase, which is the major active transport system responsible for the electrochemical potential in mammalian cells, can therefore be an indication of the effect that a chemical may have on the viability of the cell membrane and possibly the whole cell. - [Read Hamster Ovary Cell NA+/K+ -ATPase Test]

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Using confocal laser-scanning microscope & GFP fusion proteins in time-lapse imaging to visualize the behavior of organelles and to track membrane-bound transport intermediates that bud off from organelles. Practical issues related to construction & expression of GFP fusion proteins are discussed. Essential for optimizing the brightness and expression levels of GFP fusion proteins so that intracellular membrane-bound structures containing these fusion proteins can be readily visualized. - [Read Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells]

Category: Protein Protocols: Protein Trafficking

Import assays into chloroplasts. Includes: Preparation of chloroplasts and lysates; Transport of lumenal proteins. - [Read Import Assays Into Chloroplasts: Integration or Transport into Thylakoids]

Category: Yeast Protocols

Protocol for in vitro endoplasmic reticulum to golgi transport reaction in Yeast. Includes: Preparation of Membranes; One-Stage Reaction; Two-Stage Reaction using Normal Amount of Membranes; Two-Stage Reaction using Low Concentration of Membranes. - [Read In Vitro Endoplasmic Reticulum to Golgi Transport Reaction in Yeast Protocol]

Category: Yeast Protocols

Protocol for microsome high speed pellet and cytosol preparation for endoplasmic reticulum to golgi transport assay in Yeast. - [Read Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport]

Category: Cell Organelle Protocols: Microsome Protocols

Protcol relating to microsome high speed pellet and cytosol preparation for endoplasmic reticulum to golgi transport assay in yeast. Includes spheroplast formation. - [Read Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport Ass]

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol includes information on: Preparation of Membranes; One-Stage Reaction; Two-Stage Reaction using Normal Amount of Membranes; Two-Stage Reaction using Low Concentration of Membranes. - [Read Protcol for In Vitro Endoplasmic Reticulum to Golgi Transport Reaction in Yeast]

Category: Cell Biology and Cell Culture: Endothelial Cell Protocols

Protocol for primary cultures of HUVECs. Includes: Buffers and reagents (Fetal calf serum, Phosphate Buffer Saline (PBS), Collagenase, Buffer for conservation and transport of umbilical cords, Culture medium); Cell culture. - [Read Protocol for Primary Cultures of HUVECs]

Category: Protein Protocols: Protein Trafficking

Background and methods to study plant transport. Includes new methods to study protein trafficking in plant cells, includes: Identification of protein sorting pathways in non purified samples; Localization of organelle proteins by isotype tagging/isotype-coded affinity tag; Coupling of chemical genomics and proteomics; Top down mass spectrometry; Compartment-specific markers to aid in the purification of organelles. - [Read Understanding Protein Trafficking in Plant Cells Through Proteomics]