trafficking Protocols

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Affinity Purification of Antibodies - http://cmgm.stanford.edu/~vesicle/protocols/affinity.html

Category: Protein Protocols: Antibody Purification Protocols

Affinity Purification of Antibodies. Vesicle Trafficking. - [Read Affinity Purification of Antibodies]

GST Fusion Protein Prep - http://cmgm.stanford.edu/~vesicle/protocols/gst_prot.html

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Detailed protocols and methods for GST protein purification. Vesicle Trafficking, Stanford. - [Read GST Fusion Protein Prep]

Colony Hybridization Protocol - http://cmgm.stanford.edu/~vesicle/protocols/hybrid.html

Category: RNA Protocols: cDNA Libraries Library

Methods and procedure for Colony Hybridization. Vesicle Trafficking Home. - [Read Colony Hybridization Protocol]

Monster Green® Fluorescent Protein Assay - http://www.promega.com/catalog/country_select.asp?/tbs/tb320/tb320.html&ckt=2

Category: Reporter Gene Assays: GFP Assay Protocols

Green fluorescent protein is commonly used to monitor gene expression and protein trafficking within intact cells. The Monster Green® Fluorescent Protein is encoded by an improved synthetic version of the green fluorescent protein gene originally cloned from Montastrea cavernosa (Great Star Coral). - [Read Monster Green® Fluorescent Protein Assay]

Analysis of Membrane Trafficking and Intracellular Signaling in Self-Generated Iodixanol Gradients - http://www.axis-shield.com/densityhome/optiprep/S25.pdf

Category: Protein Protocols: Protein Trafficking

Protocol is based on methods for the resolution of GLUT4 containing vesicles and the identification of phosphoinositide kinase containing vesicles in 3T3-L1 adipocytes. They may have a wider application to any low-medium density membranes. Protocol incorporates the strategy of using a low density microsome fraction as the gradient input, commonly used in GLUT 4 studies that may have a wider application to other investigations. - [Read Analysis of Membrane Trafficking and Intracellular Signaling in Self-Generated Iodixanol Gradients]

Protein Trafficking Methods - http://mips.gsf.de/proj/eurofan/eurofan_2/n5/#Methods

Category: Protein Protocols: Protein Trafficking

Methods on protein trafficking. Information on N5 Secretion and protein trafficking. Methods include: Alcian Blue Test; Lucifer Yellow uptake assay; Preparation of total protein extracts for western immunoblots; Screen for Drug Sensitivity. - [Read Protein Trafficking Methods]

Membrane Trafficking and Organelle Reagents - http://www.cshprotocols.org/cgi/content/extract/2006/29/pdb.ip23

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Several common drugs, their targets, and protocols are described for studying organelle distribution and trafficking. The drugs are readily available from general suppliers, including Sigma, Roche, and Calbiochem. - [Read Membrane Trafficking and Organelle Reagents]

Understanding Protein Trafficking in Plant Cells Through Proteomics - http://www.cepceb.ucr.edu/resources/pdf/pan/ERP-review2_781_2005.pdf

Category: Protein Protocols: Protein Trafficking

Background and methods to study plant transport. Includes new methods to study protein trafficking in plant cells, includes: Identification of protein sorting pathways in non purified samples; Localization of organelle proteins by isotype tagging/isotype-coded affinity tag; Coupling of chemical genomics and proteomics; Top down mass spectrometry; Compartment-specific markers to aid in the purification of organelles. - [Read Understanding Protein Trafficking in Plant Cells Through Proteomics]