Topo Cloning procedure for cloning of small amounts DNA fragments by PCR. Cloning the PCR fragment into the TOPO vector, transforming E. Coli cells, and using blue/white selection to determine transformants. Culture inoculation, Qiagen Plasmid Prep protocol for plasmid isolation of the plasmid containing the cloned copies. Preuss Lab, Univ. Chicago. - [Read Topo Cloning Procedure]
Zero Blunt TOPO PCR Cloning Kit, Detailed protocol.
(Invitrogen Life technologies). Alam Lab. Department of Microbiology, University of Hawai'i. - [Read Zero Blunt TOPO PCR Cloning Kit Protocol]