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A high yield affinity purification method for specific RNA-binding proteins. - http://www.pubmedcentral.gov/pagerender.fcgi?tool=pmcentrez&pageindex=1&artid=330202

Category: RNA Protocols: RNA-binding Protein Purification

A high yield affinity purification method for specific RNA-binding proteins: isolation of the iron regulatory factor from human placenta. Neupert 1990. - [Read A high yield affinity purification method for specific RNA-binding proteins.]

AFLP: a new technique for DNA fingerprinting. - http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=7501463

Category: PCR Protocols: AFLP PCR

P Vos, R Hogers, M Bleeker, M Reijans, T van de Lee, M Hornes, A Frijters, J Pot, J Peleman, and M Kuiper. 1995. Nucleic Acid Research. The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic - [Read AFLP: a new technique for DNA fingerprinting.]

Annexin V Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/chap16.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

Annexin V, belonging to a recently discovered family of proteins, the annexins, with anticoagulant properties has proven to be a useful tool in detecting apoptotic cells since it preferentially binds to negatively charged phospholipids like PS in the presence of Ca2+ and shows minimal binding to phosphatidylcholine and sphingomyeline. Changes in PS asymmetry, which is analyzed by measuring Annexin V binding to the cell membrane, were detected before morphological changes associated with... - [Read Annexin V Protocol]

Antibiotics. and Antibiotics Stock Calculator Tools - http://molbiol.edu.ru/eng/protocol/01_05.html

Category: Microbiology: Antibiotics Concentration in Media LB

Working concentrations and stock solutions and which antibiotics are light sensitive. Preparation of stock solutions and calculator tool to calculate concentrations for stock solutions. Practical Molecular Biology. - [Read Antibiotics. and Antibiotics Stock Calculator Tools]

Antibody Microarrays Are a Powerful Tool for Proteomics - http://www.genpromag.com/ShowPR.aspx?PUBCODE=018&ACCT=1800000100&ISSUE=0505&RELTYPE=PR&ORIGRELTYPE=MIC&PRODCODE=00000000&PRODLETT=Z

Category: Antibody Microarray Protocols

Antibody Microarrays Are a Powerful Tool for Proteomics. Genomics and Proteomics - [Read Antibody Microarrays Are a Powerful Tool for Proteomics]

Beckman Rotor Calculator Tool Converter - http://www.beckman.com/resourcecenter/labresources/centrifuges/rotorcalc.asp

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Beckman Rotor Calculator Tool and Converter. RPM to G's. Rotor calculations. Radius, RCF. - [Read Beckman Rotor Calculator Tool Converter]

Blue-Native PAGE in Plants: A Tool in Analysis of Protein-Protein Interactions - http://www.plantmethods.com/content/1/1/11

Category: Plant Biology Protocols: Plant Proteins, Peptides and Antigens

BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science. - [Read Blue-Native PAGE in Plants: A Tool in Analysis of Protein-Protein Interactions]

Buffer calculator tool - http://molbiol.ru/eng/protocol/01_07.html

Category: Buffers Media, and Solutions

Buffer calculator tool for commonly used buffer compound solutions. Making different volumes? use this! (Practical Molecular Biology) - [Read Buffer calculator tool]

Buffers for pH Control. Calculator tool. - http://www.bioinformatics.org/JaMBW//5/4/index.html

Category: Buffers Media, and Solutions

Buffers for pH Control. Calculator tool. Rob Beynon, 1996. - [Read Buffers for pH Control. Calculator tool.]

cDNA / AFLP - http://www.dpw.wau.nl/pv/aflp/cDNA-AFLP%20Protocol.htm

Category: Genetics and Genomics: Cancer Genetics Protocols

CDNA/AFLP. A tool for transcriptome analysis. - [Read cDNA / AFLP]

Cell Staining for Sorting of Hematopoietic Stem Cells (HSC) and Myeloid Progenitors - http://www.natureprotocols.com/2006/06/29/cell_staining_for_sorting_of_h.php

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

The combination of prospective identification/isolation of bone marrow progenitors and quantitative RT-PCR is a powerful tool to understand the molecular mechanism underlying hematopoiesis. Describes the standard procedures of the murine myeloid progenitor staining for fluorescence activated cells sorting (FACS) and RNA purification methods. - [Read Cell Staining for Sorting of Hematopoietic Stem Cells (HSC) and Myeloid Progenitors]

Cell Staining for Sorting of Hematopoietic Stem Cells and Myeloid Progenitors and Isolating RNA - http://www.natureprotocols.com/2006/06/29/cell_staining_for_sorting_of_h.php

Category: Stem Cell Protocols

The combination of prospective identification/isolation of bone marrow progenitors and quantitative RT-PCR is a powerful tool to understand the molecular mechanism underlying hematopoiesis. Here, we described our standard procedures of the murine myeloid progenitor staining for fluorescence activated cells sorting (FACS) and RNA purification methods. - [Read Cell Staining for Sorting of Hematopoietic Stem Cells and Myeloid Progenitors and Isolating RNA]

DNA-Binding Protein Purification with Dynabeads. - http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2671937

Category: DNA Protocols: EMSA DNA-Protein Protocols

Magnetic DNA affinity purification of yeast transcription factor tau--a new purification principle for the ultrarapid isolation of near homogeneous factor. Gabrielsen et al. 1989 - [Read DNA-Binding Protein Purification with Dynabeads.]

Hairpin-bisulfite PCR: Assessing epigenetic - http://staff.washington.edu/miner/hpbspcr/Laird%20et%20al%202004.pdf

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Hairpin-bisulfite PCR will be a powerful tool in studying other processes for which genetic or epigenetic information differs on the two complementary strands of DNA. Laird et al., 2003. PNAS - [Read Hairpin-bisulfite PCR: Assessing epigenetic]

Imaging Hoechst-Labeled Chromosomes and Fluorescent Proteins during the Cell Cycle - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4673

Category: Microinjection Protocols

Fluorescence microscopy provides a powerful tool for imaging molecular components in living cells. Specific molecular components can be efficiently labeled by a combination of three methods: chemical transfection of GFP-fusion constructs, staining of chromosomes with the DNA-specific, fluorescent dye Hoechst 33342, and microinjection of fluorescently conjugated proteins. This procedure provides an example of using all three methods in sequence to label components of living HeLa cells. - [Read Imaging Hoechst-Labeled Chromosomes and Fluorescent Proteins during the Cell Cycle]

Introduction to Live-Cell Imaging Techniques - http://microscopy.fsu.edu/primer/techniques/livecellimaging/index.html

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Live-cell imaging techniques provide critical insight into the fundamental nature of cellular & tissue function, especially due to the rapid advances that are currently being witnessed in fluorescent protein & synthetic fluorophore technology. Because of these advances, live-cell imaging has become a requisite analytical tool in most cell biology labs. Includes: Maintaining Live Cells on the Microscope Stage; Live-Cell Imaging Culture Chambers; Optical System and Detector Requirements etc. - [Read Introduction to Live-Cell Imaging Techniques]

Karyotyping Protocol - http://humgen.wustl.edu/hdk_lab_manual/cam/cam5.html

Category: Cytogenetics Protocols

Karyotyping is a valuable research tool used to determine the chromosome complement within cultured cells. It is important to keep in mind that karyotypes evolve with continued culture. Because of this evolution, it is important for the interpretation of biochemical or other data, that the karyotype of a specific sub-line be determined. - [Read Karyotyping Protocol]

Maintaining Live Cells on the Microscope Stage - http://www.microscopyu.com/articles/livecellimaging/livecellmaintenance.html

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Live-cell imaging techniques provide a critical insight into the fundamental nature of cellular and tissue function, especially due to the rapid advances that are currently being witnessed in fluorescent protein and synthetic fluorophore technology. Because of these advances, live-cell imaging has become a requisite analytical tool in most cell biology laboratories. - [Read Maintaining Live Cells on the Microscope Stage]

Metabolic Labeling of Plant Cell Cultures with K15NO3 Protocol - http://www.plantmethods.com/content/2/1/14

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

Protocol for metabolic labeling of plant cell cultures with K15NO3 as a tool for quantitative analysis of proteins and metabolites. - [Read Metabolic Labeling of Plant Cell Cultures with K15NO3 Protocol]

Original Real Time Paper. Allelic discrimination by nick-translation PCR with fluorogenic probes. - http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8367293

Category: Real Time PCR: Real Time PCR Information

Allelic discrimination by nick-translation PCR with fluorogenic probes. Lee et al., 1993. - [Read Original Real Time Paper. Allelic discrimination by nick-translation PCR with fluorogenic probes.]

Spectrophotometric Measurement of Nucleic Acids' Concentration Tool - http://molbiol.ru/eng/scripts/01_03.html

Category: General Research Protocols and Methods: Spectrophotometry Protocols

Spectrophotometric Measurement of Nucleic Acids' Concentration Tool. This bioinformatic program help calculate the concentration of nucleic acids according to optical density (including DNA, RNA, oligonucleotides). Zbio - [Read Spectrophotometric Measurement of Nucleic Acids' Concentration Tool]

The Whole-Cell Patch-Clamp Technique â€“ a powerful tool to approach CFTR function - http://pen2.igc.gulbenkian.pt/cftr/vr/d/hug_whole_cell_patch_clamp_tool_cftr_function.pdf

Category: Electrophysiology Ion Channels

The Whole-Cell Patch-Clamp Technique â€“ a powerful tool to approach CFTR function. Martin J. Hug. University of Muenster, Institute of Physiology. Germany - [Read The Whole-Cell Patch-Clamp Technique â€“ a powerful tool to approach CFTR function]

Tricineâ€“SDS-PAGE Protocol PDF - http://www.nature.com/nprot/journal/v1/n1/pdf/nprot.2006.4.pdf

Category: Protein Protocols: Protein Electrophoresis

Tricineâ€“SDS-PAGE Protocol and background. Nature. PDF file. Tricineâ€“SDS-PAGE is commonly used to separate proteins in the mass range 1â€“100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. â€“SDS-PAGE is also used preferentially for doubled SDS-PAGE (dSDS-PAGE), a proteomic tool used to isolate extremely hydrophobic proteins for mass spectrometric identification. - [Read Tricineâ€“SDS-PAGE Protocol PDF]

Use of PCR to Prepare a Double-Stranded DNA Library Encoding Random Peptides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4135

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

PCR is used as a preparative tool for the synthesis of a high-complexity double-stranded DNA library. In the example presented here, a mixture of synthetic oligonucleotides is used to synthesize a random peptide NNK library, where K is either T or G. The exclusion of A and C nucleotides at the third position decreases the occurrence of stop codons but still allows codons for all 20 amino acids. - [Read Use of PCR to Prepare a Double-Stranded DNA Library Encoding Random Peptides Protocol]

Articles (1-4 of 4)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

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