titration Protocols

Category: Immunology: T Cell Protocols

Protocol for the Stimulation of human peripheral blood mononuclear cells with anti-human CD3 monoclonal antibody; MTT assay for detection of cellular proliferation. Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. Performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. - [Read Human T Cell Activation Protocol]

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol for plaque assays for adenovirus titration. - [Read Plaque Assays for Adenovirus Titration Protocol]

Category: Chromatography Protocols: Ion Exchange Chromatography Protocols

Protocol for selection of an ion exchanger: Determining the pI of a protein using the titration curve method. The choice of whether to use an anion or a cation exchanger should be based on knowledge of the stability of the protein, and the binding properties of the target protein and other molecules present in the sample. - [Read Selection of an Ion Exchanger Protocol II]

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Titration of DNaseI for nick translation. Sanger Institute. - [Read Titration of DNaseI for nick translation]

Category: Viral Protocols

Protocol for the titration of retrovirus. - [Read Titration of Retrovirus Protocol]