titer Protocols

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ELISA Procedure for Measuring Serum Antibody Titer - http://www.komabiotech.com/technical/protocol/ELISA.htm

Method and ELISA Procedure for Measuring Serum Antibody Titer. Komabiotech - [Read ELISA Procedure for Measuring Serum Antibody Titer]

Polyclonal Antibody Production Protocol - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Immunology/PAb.html

Category: Antibody Protocols: Antibody Production Protocols

Protocol for polyclonal antibody production. Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Includes: Rabbit Immunization; Initial Preparation; Pre-bleed; Antigen Injection; Monitoring of Titer; Purification of Antibodies. - [Read Polyclonal Antibody Production Protocol]

Amplification of Baculovirus Plaques and Preparation of High Titer Virus Stocks Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p608

Category: Cloning Protocols: Baculovirus Protein Expression Protocols

Protocol for amplification of Baculovirus plaques and preparation of high titer virus stocks. Includes: Primary Amplification; Secondary Amplification. - [Read Amplification of Baculovirus Plaques and Preparation of High Titer Virus Stocks Protocol]

Automated CellTiter-Blue Cell Viability Assay Protocol - http://www.promega.com/tbs/ep015/ep015.pdf

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol on automated cell titer- blue cell viability assay. - [Read Automated CellTiter-Blue Cell Viability Assay Protocol]

Phage Titer - http://a32.lehman.cuny.edu/molbio_course/titer.htm

Category: Molecular Biology Protocols: Phage Protocols and Methods

Plating Lambda Phage to Generate Plaques. Elution of Phage. Phage DNA Extraction. Dr.Wurtzel. Dept. Biological Sciences, Lehman College. - [Read Phage Titer]

Automated CellTiter-Glo Luminescent Cell Viability Assay Protocol - http://www.promega.com/tbs/ep014/ep014.pdf

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol for the automated cell titer- glo luminescent cell viability assay. - [Read Automated CellTiter-Glo Luminescent Cell Viability Assay Protocol]

A Simplified System for Rapid Generation of Recombinant Adenoviruses - http://www.coloncancer.org/adeasy/protocol.htm

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

A simplified system for rapid generation of recombinant adenoviruses. Includes: Generation of Recombinants in Bacterial Cells; Viral Production in 293 or 911 Cells; Preparation of High Titer Viral Stocks. - [Read A Simplified System for Rapid Generation of Recombinant Adenoviruses]

CellTiter-Glo Luminescent Cell Viability Assay Protocol - http://www.promega.com/tbs/tb288/tb288.pdf

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol for cell titer- glo luminescent cell viability assay. - [Read CellTiter-Glo Luminescent Cell Viability Assay Protocol]

Clone Genes From a Phage Library Protocol - http://www.ucsf.edu/micro/faculty/Johnson/protocols/protocol1.html

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

Protocol for cloning genes from a phage library. Includes: Titer and plate out phage; Lift plaques onto filters and prepare them for screening; Make a probe; Hybridize the probe to the filters; Wash the filters and expose to film; Purify putative plaques; Excise plasmid from the desired phage. - [Read Clone Genes From a Phage Library Protocol]

Preparation of High Titer Adenovirus in P11 Cells Protocol - http://www.whitelabs.org/Lab%20Protocols/adenovirus%20protocols/Preparation%20of%20High%20Titer%20Adenovirus%20in%20P11%20cells.htm

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol for the preparation of high titer adenovirus in P11 cells. - [Read Preparation of High Titer Adenovirus in P11 Cells Protocol]

Screening a cDNA Library for use with HybriZAP zebrafish cDNA libraries - http://www.cbs.umn.edu/~kclark/protocols/library.html

Category: RNA Protocols: cDNA Libraries Library

Procedures: Titer the library to determine the concentration of infectious phage, Plate and lift the phage particles, Hybridize the immobilized cDNA with labeled probe, Pick positive plaques. Karl Clark U. Minn. - [Read Screening a cDNA Library for use with HybriZAP zebrafish cDNA libraries]

Producing Single-stranded DNA with Phagemid Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4019

Category: Viral Manipulation Protocols: Phage Protocols

Protocol describes methods to superinfect bacteria carrying a recombinant phagemid with a high-titer stock of an appropriate helper virus and to assay the yield of filamentous virus particles that carry single-stranded copies of the phagemid DNA. The key to success in using phagemids is to prepare a stock of helper virus whose titer is accurately known. - [Read Producing Single-stranded DNA with Phagemid Vectors Protocol]

Preparing Stocks of Bacteriophage {lambda} by Small-scale Liquid Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3971

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

High-titer stocks of bacteriophage {lambda} are easily prepared by infecting small-scale bacterial cultures. - [Read Preparing Stocks of Bacteriophage {lambda} by Small-scale Liquid Culture Protocol]

Placenta Specific Gene Manipulation by Transducing Zona-Free Blastocyst using Lentiviral Vector - http://www.natureprotocols.com/2007/01/24/placenta_specific_gene_manipul.php

Category: Cytogenetics Protocols

Details a placenta specific gene manipulation by transducing blastocysts with lentiviral vectors1. After a removal of zona pellucida which functions as a physical barrier, trophoblast cells lying outermost layer of blastocyst were transduced from outside with high-titer lentiviral vectors. As most placental cells descend from trophoblast cells while fetus originated from inner cell mass, transgene expression can be observed in trophoblast cells from preimplantation stages and in placenta... - [Read Placenta Specific Gene Manipulation by Transducing Zona-Free Blastocyst using Lentiviral Vector]

Articles (1-3 of 3)

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.