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Neuroscience Histology Methods - http://psych.colorado.edu/~dbarth/PDFs/4052/4052%20Manual%20Chapters/Histology%20I.pdf

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Materials for cresyl violet staining protocol (see methods section) ... Histology also refers to the techniques used to prepare tissue for microscopic study ... Neuroscience Histology Methods - [Read Neuroscience Histology Methods]

Northern blot Protocol for the Detection of RNA in Neurospora - http://www.fgsc.net/neurosporaprotocols/Northern%20edited%20version.pdf

Category: Fungi Protocols: Neurospora Protocols

Northern blot protocol for the detection of RNA in Neurospora. Includes: Extract RNA from tissue powder; Electropheresis and transfer RNA; Preparing the membrane for probing and preparation of a riboprob.; Hybridization. - [Read Northern blot Protocol for the Detection of RNA in Neurospora]

Open Directory - Science: Biology: Botany: Tissue Culture - http://dmoz.org/Science/Biology/Botany/Tissue_Culture/

Category: Plant Biology Protocols: Plant Tissue Culture

Plant Tissue Culture - Laboratory protocol for micropropagation from stem tip, node, meristem, embryo or seed. Includes forum maintained by the National ... - [Read Open Directory - Science: Biology: Botany: Tissue Culture]

Optimized Protocol for Mounting Tissue Sections PDF - http://arctur.com/research_portal/images/Application%20Notes/pen_membrane_protocol_9.pdf

Category: Histology Protocols: Tissue Sample Sectioning

Generally Required Materials and Equipment for Tissue Sectioning. Sectioning and Slide Preparation Procedure onto Metal-Framed PEN Membrane Slides. Arcturus. - [Read Optimized Protocol for Mounting Tissue Sections PDF]

Paraffin Sectioning for LCM Plant Tissue - http://schnablelab.plantgenomics.iastate.edu/docs/resources/protocols/Paraffin_sectioning_for_LCM.pdf

Category: Histology Protocols: Tissue Sample Sectioning

FIXATION, DEHYDRATION, PARAPLAST INFILTRATION AND SECTIONING PROTOCOL WITH TISSUE COLLECTION PROCEDURE USING THE PALM LASER MICRODISSECTION SYSTEM. Schnable Labs, Plant Genomics. - [Read Paraffin Sectioning for LCM Plant Tissue]

Partial Mouse Hepatectomy Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4384

Category: Mouse Protocols: Mouse Organ Removal Surgery Protocols

Protocol describes partial removal of a mouse liver to provide tissue for analysis. - [Read Partial Mouse Hepatectomy Protocol]

PCR from Tissue - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/PCR/directTissuePCR.html

Category: Plant Biology Protocols: Plant PCR

PCR from Tissue - [Read PCR from Tissue]

PCR From TIssue Protocol - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/PCR/directTissuePCR.html/

Category: Plant Biology Protocols: Plant PCR

Protocol on how to perform a PCR from plant tissue (leaf or flower). - [Read PCR From TIssue Protocol]

PCR of Blood, Hair or Small Tissue Samples of Mouse Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp8.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for the PCR of blood, hair or small tissue samples of mouse. Includes: Preparation of blood or a tissue sample obtained by ear punch for PCR; Preparation of hair shafts for PCR. - [Read PCR of Blood, Hair or Small Tissue Samples of Mouse Protocol]

Phenol Extraction of rRNA - http://homepages.gac.edu/~cellab/chpts/chpt14/ex14-7.html

Category: RNA Protocols: rRNA Extraction and Isolation

Phenol Extraction of rRNA from Rat Liver Tissue. Dr. William Heidcamp. - [Read Phenol Extraction of rRNA]

PLANT CELL, TISSUE AND ORGAN CULTURE - 1998 - http://www.hort.purdue.edu/hort/courses/HORT515/Callus-Ref.html

Category: Plant Biology Protocols: Plant Tissue Culture

Dodds, JH and LW Roberts 1995 Experiments in Plant Tissue Culture. ... 19-60 In Plant Cell Culture Protocols, Humana Press, Totowa ... - [Read PLANT CELL, TISSUE AND ORGAN CULTURE - 1998]

Plant tissue culture - http://www.oup.com/uk/orc/bin/9780199254682/ch02.pdf

Category: Plant Biology Protocols: Plant Tissue Culture

Integration of plant tissue culture into plant. transformation protocols. Various methods of plant regeneration are available to the plant biotechnol- ... - [Read Plant tissue culture]

Plant Tissue Culture Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Media_Preparation.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for plant tissue culture media. Includes: Preparation from Packaged Powder; Preparation from Basal Salt Solutions. - [Read Plant Tissue Culture Media Protocol]

Plant Tissue Culture Protocols. Sigma - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols.html

Category: Cell Biology and Cell Culture

Plant Tissue Culture Protocols. Sigma - [Read Plant Tissue Culture Protocols. Sigma]

Plant Tissue Culture Techniques - http://www.zoo.utoronto.ca/able/volumes/vol-11/9-mineo.pdf

Category: Plant Biology Protocols: Plant Tissue Culture

Plant tissue culture techniques. Pages 151-174, in Tested studies for laboratory ... engineering protocols employ callus initiation procedures after DNA has ... Mineo Zoology Univ. Toronto - [Read Plant Tissue Culture Techniques]

Preparation and LCM of Paraffin Embedded Tissue Sections Protocol - http://dir.nichd.nih.gov/lcm/LCMTAP.htm#II.%20Preparation%20and%20LCM%20of%20Paraffin%20Embedded%20Tissue%20Sections

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Protocol for the preparation of LCM of Paraffin embedded tissue sections. Includes: Fixation; Processing, Embedding and Tissue Sectioning; Staining; Laser Transfer; DNA Extraction Protocol of LCM tissue and PCR for DNA analysis; RNA Extraction Protocol of LCM tissue and RT-PCR for mRNA analysis. - [Read Preparation and LCM of Paraffin Embedded Tissue Sections Protocol]

Preparation of Cells and Reagents for Flow Cytometry Protocols - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C3CC685613447CEC768412FDE0F&objectid=6674EA50F5C3D9A0189B376C7A89BF6B

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

A procedure for direct and indirect staining of single-cell suspensions of lymphoid tissue or peripheral blood lymphocytes to detect cell surface membrane antigens is presented. In addition, support protocols present methods for fluorescence labeling of purified antibodies. A protocol for flow cytometric analysis of intracellular antigens in single-cell suspensions is also included. - [Read Preparation of Cells and Reagents for Flow Cytometry Protocols]

Preparation of Chromosome Spreads - http://www.le.ac.uk/bl/phh4/prochrom.htm

Category: Cytogenetics Protocols

An ideal method of tissue preparation ensures both good specimen morphology and that the target molecules are in the optimum state for probe access and hybridization. DNA:DNA in situ hybridization is usually carried out on chromosome spread preparations where chromosome and nuclei are released from cells and spread on a glass microscope slide. This method yields well separated and enlarged chromosomes with good morphology which can be analyzed in transmitted light or fluorescence microscopes. - [Read Preparation of Chromosome Spreads]

Preparation of DNA from Cells in 24-Well Tissue Culture Plates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4414

Category: Stem Cell Protocols

This protocol describes a method of DNA preparation from ES cells in 24-well plates. - [Read Preparation of DNA from Cells in 24-Well Tissue Culture Plates Protocol]

Preparation of Paraffin Sections and Frozen Tissue for FISH Protocol - http://www.riedlab.nci.nih.gov/publications/2412.2845_Paraff_FrozTiss_Slide_Pretreat.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for the preparation of paraffin sections and frozen tissue for FISH. - [Read Preparation of Paraffin Sections and Frozen Tissue for FISH Protocol]

Preparation of RNA from Paraffin-Embedded Fixed Tissue Using Trizol Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4103

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

The method uses Trizol, a phenol-base reagent produced by Invitrogen. - [Read Preparation of RNA from Paraffin-Embedded Fixed Tissue Using Trizol Protocol]

Preparation of RNA from Plant Tissue Using Guanidinium Isothiocyanate/Cesium Chloride Ultracentrifug - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4102

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Tissues are homogenized in guanidinium isothiocyanate lysis buffer and then subjected to cesium chloride ultracentrifugation. The resulting RNA is then extracted with phenol and chloroform to remove protein and lipid contaminants. - [Read Preparation of RNA from Plant Tissue Using Guanidinium Isothiocyanate/Cesium Chloride Ultracentrifug]

Preparation of RNA from Plant Tissue Using Trizol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4105

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol is based on the standard Trizol protocol for the purification of RNA from animal cells using Trizol (Purification of RNA from Animal Cells using Trizol). In this version, adapted for use with plant tissues, a high-salt isopropanol precipitation step has been added to precipitate RNA selectively, while maintaining polysaccharides and proteoglycans in solution. - [Read Preparation of RNA from Plant Tissue Using Trizol]

Preparation of Solid Tissue for Aspergillus Galactomannan Antigen Detection by Platelia Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./galactoman.html~main

Category: Fungi Protocols

Protocol for the preparation of solid tissue for Aspergillus galactomannan antigen detection by Platelia (Biorad). Technique was designed for use on human serum. However, it may also be possible to perform this method on solid tissues and organic solutions. Viscous solution and tissue specimens need to be pre-treated to achieve the extraction of the Aspergillus antigen and to get a homogeneous sample in solution. - [Read Preparation of Solid Tissue for Aspergillus Galactomannan Antigen Detection by Platelia Protocol]

Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections Protocol - http://dir.nichd.nih.gov/lcm/LCMTAP.htm#I.%20Preparation,%20LCM%20and%20RNA/DNA%20extraction%20of%20Frozen%20Tissue%20Sections

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Protocol for the preparation, LCM and RNA/DNA extraction of frozen tissue sections. Includes: Embedding, Cutting and Staining. - [Read Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections Protocol]

Articles (1-10 of 11)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.