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Elastic Picro Sirius Red Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/epsr.html

Category: Neuroscience Protocols: Histology Stains Protocols: Connective Tissue Stains Protocols

Protocol for elastic picro sirius red connective tissue stain. - [Read Elastic Picro Sirius Red Stain Protocol]

Electron Microscope Lab protocols - http://biology.berkeley.edu/EML/protocols.html

Category: Molecular Imaging: Electron Microscopy Protocols

Generic processing protocol ï¿½ PLT/ LR Gold ï¿½ SEM Generic Processing protocol ï¿½ SEM Microwave tissue processing protocol ï¿½ Uranyl acetate and Lead citrate ...Electron Microscope Lab protocols at UC Berkeley - [Read Electron Microscope Lab protocols]

Enzymatic Protocol for Immunocytochemistry - http://www.rndsystems.com/ihc_detail_objectname_EnzymaticProtocol20.aspx

Category: Immunology: Immunocytochemistry Protocols

Staining Tissue, Staining Cells Smeared on Slides. Positive staining controls, Negative staining controls, Immunoenzymatic Technique and FAQ. R & D Systems. - [Read Enzymatic Protocol for Immunocytochemistry]

Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tis - http://www.jhc.org/cgi/content/full/46/3/397

Category: Protein Protocols: Protein Extraction From Tissue

Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tissue sections. Ikedaa et al, 1998 JHC. - [Read Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tis]

Extraction and Purification of RNA from Tissue Samples for Fluorescent mRNA Differential Display - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4124

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from tissue samples. - [Read Extraction and Purification of RNA from Tissue Samples for Fluorescent mRNA Differential Display]

Extraction and Purification of RNA from Tissue-Cultured Cells for Fluorescent mRNA Differential - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4123

Category: PCR Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from the tissue-cultured cells of interest. For other starting materials, such as blood samples, please see Extraction and Purification of RNA from Blood Samples for Fluorescent mRNA Differential Display. - [Read Extraction and Purification of RNA from Tissue-Cultured Cells for Fluorescent mRNA Differential]

Extraction Method for Formalin Pigments from Tissue Sections Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1015

Category: Lipid Protocols

Protocol for extraction method for formalin pigments from tissue sections. - [Read Extraction Method for Formalin Pigments from Tissue Sections Protocol]

Extraction of Calf Brain Lipids Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1281

Category: Lipid Protocols

Protocol for the extraction of calf brain lipids. Protocol describes a rapid method to isolate lipids from bovine brain tissue using an organic solvent mixture of Chloroform and Methanol. - [Read Extraction of Calf Brain Lipids Protocol]

Fixation and Fixatives - A great guide - http://home.primus.com.au/royellis/fix.htm

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Fixation is to preserve cells and tissue constituents in as close a life-like state as possible and to allow them to undergo further preparative procedures without change. Fixation arrests autolysis and bacterial decomposition and stabilises the cellular and tissue constituents so that they withstand the subsequent stages of tissue processing. Great detailed guide with protocols. Anthony S-Y Leong. - [Read Fixation and Fixatives - A great guide]

Fixation Protocol - http://cmif.osu.edu/419.cfm

Category: Histology Protocols: Fixation of Cells Tissues Protocol

There are many variations based on the type of tissue to be examined, whether one is dealing with cell suspensions, biopsies, perfused tissues, or monolayer . Fixation Protocol. OSU Campus Microscopy & Imaging Facility :: The Ohio State University College of Med - [Read Fixation Protocol]

FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING - http://www.vetmed.auburn.edu/index.pl/cell_cycle/dna_content

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Analysis of Cell Cycle Based on DNA Content. FIXATION PROTOCOL FOR FLOW CYTOMETRIC ... Liberate cells from tissue culture flask by trypsin digestion and ... FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING. Auburn University, College of Veterinary Medicine. You. - [Read FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING]

Fixing Attached Cells in Organic Solvents Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4293

Category: Cell Culture Protocols: Cell Fixing Protocols

Organic solvents such as alcohols and acetone remove lipids and dehydrate cells, precipitating the proteins on the cellular architecture. Be aware that different antigens may be affected differently by the various solvents. If no previous data are available for your antigen, start with the 50/50 mixture. For tissue culture dishes, concentrations of acetone higher than 50% will destroy the integrity of the plastic. - [Read Fixing Attached Cells in Organic Solvents Protocol]

Fluorescent In Situ Transcription in Cells and Tissues Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6639A7FEDD7A49BBC3758516F99557&objectid=6677C9E8B913BFB72E9F654025F3C39A

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Method assesses cellular mRNA transcripts in tissue sections and cell cultures using unique short anti-sense primers directed against sequences in particular protein(s). The unlabeled synthetic cDNA oligonucleotide primers are extended complementary to a sense mRNA transcript using reverse transcriptase and labeled through incorporation of a fluorescent-labeled dUTP nucleotide base. This procedure provides rapid detection of low abundance mRNA messages that can be related to other cellular.... - [Read Fluorescent In Situ Transcription in Cells and Tissues Protocol]

Fluorescent Reagents for Live Cell Imaging and Their Introduction into Cells - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.ip20

Category: Microscopy Protocols: Fluorescence Microscopy Protocols

Most biological specimens are relatively transparent, so details of internal and intracellular morphology are difficult to image in untreated living specimens using simple bright-field techniques. Fluorescence microscopy offers greater advantages and possibilities for increasing contrast and determining the specific localization of molecules in cells. Article outlines the three methods most commonly used to introduce an appropriate label into Drosophila tissue without perturbing the process. - [Read Fluorescent Reagents for Live Cell Imaging and Their Introduction into Cells]

Formaldehyde Treatment of Tissue Culture Hoods - http://www.nottingham.ac.uk/~mbzspd/methods/Formaldehyde_Treatme.html

Category: General Research Protocols and Methods: Sterile Technique

Formaldehyde sterilization of tissue culture hoods. A bit toxic however effective. Dr.Dawson, Department Biochemistry, Univ. Nottingham Medical School. - [Read Formaldehyde Treatment of Tissue Culture Hoods]

Frozen Sectioning and Tissue Sectioning for Electron Microscopy - http://www.ucsf.edu/pathol/research/morphprotocol.htm

Category: Histology Protocols: Tissue Sample Sectioning

Frozen Sectioning and Tissue Sectioning for Electron Microscopy. Includes Tissue Processing for Transmission Electron Microscopy and Tissue Preservation for Light Microscopy. University of California UCSF Dept. Pathology Research. - [Read Frozen Sectioning and Tissue Sectioning for Electron Microscopy]

FROZEN SECTIONING PROTOCOL Plant Tissue PDF - http://schnablelab.plantgenomics.iastate.edu/docs/resources/protocols/Cryosectioning_for_LCM.pdf

Category: Histology Protocols: Tissue Sample Sectioning

PREPARATION OF PLANT TISSUE FOR LASER MICRODISSECTION. Schnable Labs, Plant Genomics. - [Read FROZEN SECTIONING PROTOCOL Plant Tissue PDF]

General Overview of Histology - http://www-bioc.rice.edu/bios576/histology/histology.htm

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Fixation must convert the soluble contents of the cell (ie proteins, carbohydrates, ... http://www.protocol-online.org/prot/Histology/Fixation/ ...General Overview of Histology and Tissue Fixation. BIOS 576. Rice. - [Read General Overview of Histology]

Genetic Mapping of Arabidopsis Centromeres Using PCR-Based Markers Protocol - http://www.arabidopsis.org/cshl-course/3-genetic_mapping.html

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol for genetic mapping of Arabidopsis centromeres using PCR-based markers. Includes: DNA markers facilitate genome analysis; Centromere mapping using tetrad analysis; Preparation of DNA from frozen plant tissue. - [Read Genetic Mapping of Arabidopsis Centromeres Using PCR-Based Markers Protocol]

Getting DNA from Old Dead Stuff - http://www.uga.edu/srel/DNA_Lab/OldDNA99.rtf

Category: DNA Protocols: DNA Extraction Protocols: Extracting Ancient DNA

DNA Isolation from museum specimens: 1) what's the best tissue to sample; 2) what's the best way to get DNA out of that tissue? 1) the easiest well-dried tissue to get, and 2) the easiest extraction methods you are comfortable doing. Travis Glenn. - [Read Getting DNA from Old Dead Stuff]

Growing Adherent Cells Directly on Tissue Dishes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4290

Category: Cell Culture Protocols

For low-resolution work, cells to be used for staining can be grown directly on regular tissue-culture dishes. It is a convenient method that does not require much preparatory work. - [Read Growing Adherent Cells Directly on Tissue Dishes Protocol]

Growing Adherent Cells on Coverslips or Multiwell Slides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4289

Category: Cell Culture Protocols

Glass is an excellent substrate for most tissue-culture-adapted cells and is compatible with all fixing and staining solutions. Glass coverslips in tissue-culture dishes or in 24-well multiwell plates are suitable carriers, as are multiwell slides. For high-resolution studies, choose glass coverslips of the highest available grade; #1 or #1.5 coverslips are the appropriate thickness. - [Read Growing Adherent Cells on Coverslips or Multiwell Slides Protocol]

Growth of Primary Cell Culture and Viral Vector Handling Protocols - http://www.stanford.edu/dept/EHS/prod/researchlab/bio/docs/Palmer_Bio_protocol.pdf

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

The procedures involve the isolation and growth of primary cell cultures from rodent and human tissue as well as the use of viral vectors for the introduction and expression of mammalian genes in cells in culture and in live rodents. - [Read Growth of Primary Cell Culture and Viral Vector Handling Protocols]

GUS Reporter Gene Assay Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/GUS%20Assays.pdf

Category: Reporter Gene Assays

Protocol for GUS reporter gene assay. Includes: Protein isolation; Alternative method for small (<1g) quantities of tissue; GUS assays; Bradford protein concentration determination assays - [Read GUS Reporter Gene Assay Protocol]

Haematoxylin Van Gieson Tissue Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/hvg.html

Category: Neuroscience Protocols: Histology Stains Protocols: Hematoxylin Eosin Staining Protocols

Protocol for haematoxylin van gieson tissue stain. - [Read Haematoxylin Van Gieson Tissue Stain Protocol]

Articles (1-10 of 11)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.