timing Protocols

Category: Immunohistochemistry Protocols: Cryosectioning Protocols

Protocol for cryosectioning. While the timing of the various steps in this protocol are probably not critical, process the tissue all at once to ensure that RNA and/or proteins do not get degraded. Includes: 20% Paraformaldehyde/4% Paraformaldehyde-PBS; Sucrose/PBS. - [Read Cryosectioning Protocol]

Category: Stem Cell Protocols: Stem Cell Isolation Protocols

The starting material for de novo isolation of stem cell lines can be either normal 3.5-days post coitum (dpc) expanded blastocysts or "delayed" blastocysts. Delayed blastocysts are usually collected 4-6 days after ovariectomy. For both groups of blastocysts, tissue culture procedures are similar. The only difference is the timing of the first disaggregation, because delayed blastocysts will initially grow more slowly. - [Read De Novo Isolation of Embryonic Stem (ES) Cell Lines from Blastocysts Protocol]

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Information on processing of microdissected tissue for molecular analysis. Includes: More than 10,000 Cells; Less than 10,000 Cells; Formalin-fixed; Paraffin-embedded Tissue; Other Fixatives; Timing. - [Read Processing of Microdissected Tissue for Molecular Analysis]

Category: Yeast Protocols: Yeast Genetics Protocols: Yeast Replication Protocols

Protocol for replication timing by comparative hybridization. - [Read Replication Timing by Comparative Hybridization Protocol]

Category: Yeast Protocols: Yeast Genetics Protocols: Yeast Replication Protocols

Protocol for replication timing by density transfer. - [Read Replication Timing by Density Transfer Protocol]

Category: Yeast Protocols: Yeast Genetics Protocols: Yeast Replication Protocols

Protocol for replication timing using transient hemimethylation. Includes: Strain construction; Protocol for methylation timing; Quantitation. - [Read Replication Timing Using Transient Hemimethylation Protocol]

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Protcol for the timing of cycles. - [Read Timing of Cycles Protocol]

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Protocol for timing of cycles. - [Read Timing of Cycles Protocol]