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A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis - http://www.tigr.org/tdb/microarray/conciseguide.html

Category: DNA Microarray Protocols

Hegde et al., Array Fabrication, microarray Printing, Probe Preparation and Hybridization, RNA Extraction, RNA labeling, Data Collection, Normalization, and Analysis.Institute for Genomic Research, Rockville, MD - [Read A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis]

A Concise Guide to cDNA Microarray Analysis â€“ II PDF. - http://pga.tigr.org/PDF/BiotechniquesCookbook_II.pdf

Category: DNA Microarray Protocols

Micro Array Fabrication, Probe Preparation and Hybridization, and Data Collection, Normalization and Analysis. Hegde, et al. biotechniques 2000. - [Read A Concise Guide to cDNA Microarray Analysis â€“ II PDF.]

Amino-allyl Labeling - http://pga.tigr.org/sop/M004_1a.pdf

Category: DNA Microarray Protocols

This protocol describes the labeling of eukaryotic RNA with aminoallyl labeled nucleotides via first strand cDNA synthesis followed by a coupling of the aminoallyl groups to either Cyanine 3 or 5 (Cy 3/Cy5) fluorescent molecules. Hasseman. TIGR Microarr - [Read Amino-allyl Labeling]

Labeled Probe Hybridization Procedure - http://pga.tigr.org/sop/M005_1a.pdf

Category: DNA Microarray Protocols

This protocol describes the hybridization of a Cy labeled cDNA probe (mix of Cy3 and Cy5) onto coated slide spotted with PCR amplified cDNA. TIGR Microarray Protocols. - [Read Labeled Probe Hybridization Procedure]

MAKING MICROARRAY PRINTING PLATES (IN DMSO) - http://pga.tigr.org/sop/M003_1a.pdf

Category: DNA Microarray Protocols

This protocol describes the method for making microarray printing plates in a 96 well format. (The same procedure applies to a 384 well format as well.) Hasseman. TIGR Microarray Protocols - [Read MAKING MICROARRAY PRINTING PLATES (IN DMSO)]

MICROARRAY cDNA CLONE GROWTH AND TEMPLATE - http://pga.tigr.org/sop/M001_1a.pdf

Category: DNA Microarray Protocols

This protocol describes clone handling, plate replication, and DNA template preparation in a 96 well format. Hasseman. TIGR Microarray Protocols - [Read MICROARRAY cDNA CLONE GROWTH AND TEMPLATE]

MICROARRAY PCR, PURIFICATION, AND STORAGE - http://pga.tigr.org/sop/M002_1a.pdf

Category: DNA Microarray Protocols

This protocol describes PCR amplification of eukaryotic cDNA plasmid inserts, gel electrophoresis, purification, and storage of PCR products. Hasseman. TIGR Microarray Protocols. - [Read MICROARRAY PCR, PURIFICATION, AND STORAGE]

Two Step real time PCR protocol - http://pga.tigr.org/sop/RT-PCR.pdf

Category: Real Time PCR

Two Step real time PCR protocol. Frank. - [Read Two Step real time PCR protocol]