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How to Isolate Tightly-Coupled Mitochondria from Neurospora crassa mycelium Protocol - http://www.fgsc.net/neurosporaprotocols/How%20to%20Isolate%20Tightly-Coupled%20Mitochondria.pdf

Category: Fungi Protocols: Neurospora Protocols

Protocol on how to Isolate Tightly-Coupled Mitochondria from Neurospora crassa mycelium. A Fast and Reproducible Procedure - [Read How to Isolate Tightly-Coupled Mitochondria from Neurospora crassa mycelium Protocol]

Passage of Embryonic Stem (ES) Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4401

Category: Stem Cell Protocols

This protocol describes passage of ES cells. They should be split at 1:3 to 1:7 every 2-3 days depending on their growth rate when they reach 70% confluency. They should never be allowed to grow past 90% confluency, but rather they should form tightly packed colonies not touching each other. - [Read Passage of Embryonic Stem (ES) Cells Protocol]

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Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.