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Arabidopsis Thaliana DNA Isolation Procedure - http://wheat.pw.usda.gov/~lazo/methods/lazo/arabdna.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Procedure to isolate DNA from Arabidopsis Thaliana. - [Read Arabidopsis Thaliana DNA Isolation Procedure]

Arabidopsis thaliana DNA Isolation Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/arabdna.html

Category: Plant Biology Protocols: Arabidopsis Nucleic Acid Protocols

Protocol for Arabidopsis thaliana DNA isolation procedure. - [Read Arabidopsis thaliana DNA Isolation Protocol]

Arabidopsis transformation with Agrobacterium PDF - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/ArabidopsisTransformation.pdf

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Electrotransformation of Agrobacterium with a plasmid that has been replicating in E. coli. Growth of Arabidopsis thaliana. Arabidopsis dunking. Seed Harvesting. Plant tissue culture. Very detailed protocol. Stockinger lab. PDF - [Read Arabidopsis transformation with Agrobacterium PDF]

Chromatin immunoprecipitation protocol to analyze histone modifications in Arabidopsis thaliana - http://vkp.leeds.ac.uk/Drive/Drive/ViewFile?include=y&component=security&action=Start&nextAction=gotoDoc&doc=494824#search=%22chromatin%20immunoprecipitation%20protocol%22

Category: Epigenetics and Methylation Protocols: ChIP Chromatin Immunoprecipitation Protocols

Chromatin immunoprecipitation protocol to analyze histone modifications in Arabidopsis thaliana. Werner Aufsatz, Matzke Lab. Gregor Mendel Institute of Molecular Plant Biology, Austria. Includes Chromatin Crosslinking, Chromatin preparation, Pre-clearing and immuno precipitation (IP), Collection,washes and elution of immune complexes, Reverse crosslinking and DNA cleanup - [Read Chromatin immunoprecipitation protocol to analyze histone modifications in Arabidopsis thaliana]

Chromosome Spreads from Flower Buds of Arabidopsis thaliana Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1101

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Method is for preparing chromosomes from single flower buds of A. thaliana. It does not kill the plants allowing the determination of their chromosome number throughout development. Includes: Preparations of Arabidopsis; Preparation of Chromosome; Staining Chromosomes. - [Read Chromosome Spreads from Flower Buds of Arabidopsis thaliana Protocol]

Identifying Transformed Arabidopsis thaliana Seedlings Following Floral Dip Transformation Protocol - http://www.plantmethods.com/content/2/1/19

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Protocol for a rapid and robust method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation. - [Read Identifying Transformed Arabidopsis thaliana Seedlings Following Floral Dip Transformation Protocol]

Method for Establishment and Maintenance of Leaf and Root Cell Cultures Arabidopsis thaliana - http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb19/r01-030.pdf

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

Method for Establishment and Maintenance of Leaf and Root Cell Cultures Arabidopsis thaliana. CL ENCINA1, M CONSTANTIN2 and J BOTELLA2. Plant Molecular Biology Reporter 19: 245â€“248, 2001. - [Read Method for Establishment and Maintenance of Leaf and Root Cell Cultures Arabidopsis thaliana]

Plant DNA Extraction Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplmy.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

A. thaliana has a very small haploid genome and this makes obtaining DNA somewhat difficult. The most notable problem is that DNA is usually contaminated with polysaccharide which inhibit restriction enzymes as well as other DNA modifying enzymes. This problem is most easily solved by using young plants which have not accumulated as much polysaccharide as older plants. The best results are obtained with plants that are two to three weeks post germinated. - [Read Plant DNA Extraction Protocol]

Precision Engineering of Plant Gene Loci by Homologous Recombination Cloning in E coli Protocol - http://www.plantmethods.com/content/1/1/6

Category: E Coli Protocols: E coli Genetics Protocols

Protocol for precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli. Describe the basis for homologous recombination cloning in E. coli, the available tools and resources, together with a protocol for long range cloning and manipulation of an Arabidopsis thaliana gene locus, to create constructs co-ordinately driven by locus-specific regulatory elements. - [Read Precision Engineering of Plant Gene Loci by Homologous Recombination Cloning in E coli Protocol]

Precision Engineering of Plant Gene Loci by Homologous Recombination Cloning in Escherichia Coli - http://www.plantmethods.com/content/1/1/6

Category: Plant Biology Protocols: Plant Genetics Protocols

Describe the basis for homologous recombination cloning in E. coli, the available tools and resources, together with a protocol for long range cloning and manipulation of an Arabidopsis thaliana gene locus, to create constructs co-ordinately driven by locus-specific regulatory elements. - [Read Precision Engineering of Plant Gene Loci by Homologous Recombination Cloning in Escherichia Coli]

Simplified Arabidopsis Transformation Protocol - http://plantpath.wisc.edu/~afb/protocol.html

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Agrobacterium tumefaciens strain transformation of arabidopsis thaliana. Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign. - [Read Simplified Arabidopsis Transformation Protocol]

Vacuum-Infiltration Transformation of Arabidopsis thaliana Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/met3.html

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Protocol for vacuum-infiltration transformation of Arabidopsis thaliana. Includes: Plant Growth; Vacuum Infiltration; Selection of Putative Transformants; Kanamycin selection. - [Read Vacuum-Infiltration Transformation of Arabidopsis thaliana Protocol]

Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_220-228.pdf

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol for whole mount fluorescence in situ hybridization (FISH) of repetitive DNA sequences on interphase nuclei of the small cruciferous plant Arabidopsis thaliana. Includes: Seed sterilization and germination; Tissue fixation; Labeling of the probe DNA; Pretreatment; In situ hybridization; Pre-absorption of antibodies; Posthybridization washes; Immunocytochemical detection; Direct detection; Indirect detection; Staining and mounting; Fluorescence microscopy. - [Read Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase]