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Sandwich ELISA Procedure - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Biochemistry/sELISA.html

Category: ELISA Protocols: Sandwich ELISA Assay Protocols

Sandwich ELISA Procedure. Kitto Lab, Univ. Texas at Austin - [Read Sandwich ELISA Procedure]

Bisulfite Treatment of DNA for Methylation Analysis - http://www.mdanderson.org/departments/methylation/display.cfm?id=955D5740-37D4-444F-ACF0193F8EC3989B&method=displayFull

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Adapted from Frommer et.al. Good protocol for bisulfite treatment of DNA. Includes tips on Methylation PCR for CpG methylation analysis. University of Texas M. D. Anderson Cancer Center - [Read Bisulfite Treatment of DNA for Methylation Analysis]

Freezing and Thawing Cultured Cells - http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/freezin1.txt

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Methods for freezing and thawing cultured cells using DMSO. Dr. Allan Bradley. Texas. - [Read Freezing and Thawing Cultured Cells]

Fluorochrome Absorption and Emission Spectra - http://www.bdbiosciences.com/pharmingen/protocols/Fluorochrome_Absorption.shtml

Category: Molecular Imaging: Immunofluorescence Microscopy

Fluorochrome Absorption and Emission Spectra. BD Biosciences. Includes spectra of Allophycocyanin (APC), BD Cy-Chrome, Fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), PE-Texas Redâ„¢, Peridinin chlorophyll protein (PerCP), PerCP-Cy5.5, APC-Cy7, Texas Redâ„¢. - [Read Fluorochrome Absorption and Emission Spectra]

Freezing and Thawing of Mammalian Cell Lines - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Immunology/CellFreeze.html

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing and Thawing of Mammalian Cell Lines. For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture. Kitto Lab Texas. - [Read Freezing and Thawing of Mammalian Cell Lines]

Embryonic Stem Cell Protocol - http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/es.txt

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Embryonic Stem Cell Protocol. tips and protocol for culture. Allan Bradley Lab, Baylor College of Medicine, Houston, Texas - [Read Embryonic Stem Cell Protocol]

Methylation Specific PCR Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=0CB11381-59AE-4D90-AC0B343F2D646EDD&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Methylation Specific PCR Protocol. M.D Anderson Cancer Centre Texas. Protocol written by James Herman* - [Read Methylation Specific PCR Protocol]

Beta b-Galactosidase Color Assay for Cultured Cells - http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/betagal.txt

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Beta b-Galactosidase Color Assay for Cultured Cells. Bradley's Lab, Texas. - [Read Beta b-Galactosidase Color Assay for Cultured Cells]

Analysis of ES Cell Clones by Mini-Southern - http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/mini-sou.txt

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Analysis of ES Cell Clones by Mini-Southern. Bradley's Lab, Texas. - [Read Analysis of ES Cell Clones by Mini-Southern]

DNA-Methyltransferase Assay - Anderson Centre - http://www.mdanderson.org/departments/methylation/display.cfm?id=9B5B6082-C53A-4A86-BEE3EE149A52251E&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

M.D. Anderson Cancer Centre Texas - [Read DNA-Methyltransferase Assay - Anderson Centre]

Bisulfite-PCR for Restriction Analysis and/or Sequencing - http://www.mdanderson.org/departments/methylation/display.cfm?id=8E0AAAAC-FA42-486C-A93F60CD6E1C7E51&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Bisulfite-PCR for Restriction Analysis and/or Sequencing. Protocol written by Jean-Pierre Issa. M.D Anderson Cancer Centre Texas. - [Read Bisulfite-PCR for Restriction Analysis and/or Sequencing]

Methylated CpG Island Amplification - http://www.mdanderson.org/departments/methylation/display.cfm?id=8A198378-986B-41FD-8778FA1399FBAB4D&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: Epigenetics and Methylation Protocols

Methylated CpG Island Amplification. Protocol written by Minoru Toyota. M.D Anderson Cancer Centre Texas. - [Read Methylated CpG Island Amplification]

FEEDER PREPARATION BY GAMMA IRRADIATION - http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/gamma.txt

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

FEEDER PREPARATION BY GAMMA IRRADIATION. Bradley's Lab Texas - [Read FEEDER PREPARATION BY GAMMA IRRADIATION]

Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates - http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/freezing.txt

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates. Bradley Lab Texas. - [Read Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates]

Isolation of Primary Fibroblasts from Mouse Embryos - http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/primaryf.txt

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Isolation of Primary Fibroblasts from Mouse Embryos. Bradley's Lab, Texas. - [Read Isolation of Primary Fibroblasts from Mouse Embryos]