tetrahymena Protocols

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Freezing and Thawing Tetrahymena Cultures Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4497

Category: Tetrahymena Protozoa Protocols

The method for freezing Tetrahymena has undergone major modifications over the years. - [Read Freezing and Thawing Tetrahymena Cultures Protocol]

Growth and Concentration of Chlorogonium for Culturing Freshwater Hypotrichs Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4464

Category: Laboratory Model Organisms Protocols

Protocol describes the growth and concentration of the alga Chlorogonium elongatum as a food source for culturing freshwater hypotrichs. Most freshwater hypotrichs (including Oxytricha nova, O. fallax, and O. trifallax; Euplotes aediculatus and E. eurystomous; and Stylonychia lemnae) can be grown to high density with Chlorogonium as the food organism. A similar regimen can be used to prepare other food sources such as Tetrahymena or bacteria (e.g., Aerobacter aerogenes). - [Read Growth and Concentration of Chlorogonium for Culturing Freshwater Hypotrichs Protocol]

Induction of Conjugation in Tetrahymena Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4499

Category: Tetrahymena Protozoa Protocols

Mature Tetrahymena cells of opposite mating types are starved under appropriate salt conditions. The mating types are then combined to costimulate through cell-cell interaction. Loose pairs and then firm, irreversible pairs of cells of opposite mating types form. This method consistently results in a high percentage of pairing (usually greater than 80%) and good synchrony. - [Read Induction of Conjugation in Tetrahymena Protocol]

Isolation and Purification of Tetrahymena Nuclei Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4500

Category: Tetrahymena Protozoa Protocols

Protocol describes how to allow the isolation of nuclei from all stages of the Tetrahymena life cycle in high yield with a high degree of purity. This method gives highly purified populations of both micronuclei and macronuclei. - [Read Isolation and Purification of Tetrahymena Nuclei Protocol]

Long-Term Storage of Unfrozen Tetrahymena Cultures in Soybean Medium Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4472

Category: Tetrahymena Protozoa Protocols

Long-term storage of unfrozen Tetrahymena is convenient when a laboratory is not using the cells frequently; however, cells maintained under these conditions often take a couple of days of incubation in culture medium to recover. - [Read Long-Term Storage of Unfrozen Tetrahymena Cultures in Soybean Medium Protocol]

Long-Term Storage Storage of Unfrozen Tetrahymena Cultures in Maintenance Culture Medium Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4471

Category: Tetrahymena Protozoa Protocols

Protocol provides a method for long-term storage of Tetrahymena cultures using maintenance culture medium. - [Read Long-Term Storage Storage of Unfrozen Tetrahymena Cultures in Maintenance Culture Medium Protocol]

Short-Term Storage of Tetrahymena Cultures Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4470

Category: Tetrahymena Protozoa Protocols

A number of methods can be used for storage of unfrozen Tetrahymena cultures in the laboratory. Cells that are maintained using the short-term storage are described in this protocol. - [Read Short-Term Storage of Tetrahymena Cultures Protocol]

Tetrahymena Thermophila Ocular Irritancy Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/22.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Microorganism Cytotoxicity Assays Protocols

The basis of this test is that a cytotoxic chemical (regardless of site or mechanism of action) will interfere with the normal motility of the protozoan, Tetrahymena thermophila, in culture. The degree of interference of motility as compared to control cultures, related to the concentration of the test compound, provides an indication of toxicity. - [Read Tetrahymena Thermophila Ocular Irritancy Test]

Transformation of Tetrahymena thermophila by Electroporation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4502

Category: Tetrahymena Protozoa Protocols

Protocol describes a method for transformation of the Tetrahymena using electroporation. The vector is electroporated into cells after mating, where it is incorporated into the DNA of developing macronuclei. Because T. thermophila can be propagated indefinitely without conjugation, transformation of the macronucleus provides a way to obtain stable somatic transformants. DNA vectors transformed using this protocol include those containing drug-resistant versions of Tetrahymena genes. - [Read Transformation of Tetrahymena thermophila by Electroporation Protocol]

Transformation of Tetrahymena thermophila by Electroporation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4502

Category: Transfection Protocols: Electroporation Sonoporation Protocols

Protocol describes a method for transformation of the Tetrahymenausing electroporation. The vector is electroporated into cellsafter mating, where it is incorporated into the DNA of developingmacronuclei. Because T. thermophila can be propagated indefinitelywithout conjugation, transformation of the macronucleus providesa way to obtain stable somatic transformants. - [Read Transformation of Tetrahymena thermophila by Electroporation Protocol]