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A Sealed Preparation for Long-Term Observations of Cultured Cells - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4660

Category: Microscopy Protocols: Live-Cell Imaging Protocols

This protocol describes a sealed preparation that allows the continuous long-term observation of cultured mammalian cells on upright or inverted microscopes without environmental CO2 control. The preparation allows for optical conditions consistent with high-quality imaging and good cell viability for at least 100 hours. - [Read A Sealed Preparation for Long-Term Observations of Cultured Cells]

A Sealed Preparation for Long-Term Observations of Cultured Cells: Support Slide Construction - http://www.cshprotocols.org/cgi/content/extract/2007/1/pdb.ip26

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Protocol on the details required of support slide construction for a sealed preparation for long-term observations of cultured cells. - [Read A Sealed Preparation for Long-Term Observations of Cultured Cells: Support Slide Construction]

Aseptic Technique for Cell Culture Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B7EFFEB261151E9039B66FD5981&objectid=66739FA0D0CABBF1C466C9F466ABDF9A

Category: Cell Culture Protocols: Sterile Technique Protocols

This unit describes some of the ways that a laboratory can deal with the constant threat of microbial contamination in cell cultures. A protocol on aseptic technique is described first. This catch-all term universally appears in any set of instructions pertaining to procedures in which noncontaminating conditions must be maintained. - [Read Aseptic Technique for Cell Culture Protocol]

Freezing and Thawing of Mammalian Cell Lines - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Immunology/CellFreeze.html

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing and Thawing of Mammalian Cell Lines. For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture. Kitto Lab Texas. - [Read Freezing and Thawing of Mammalian Cell Lines]

Freezing and Thawing of Mammalian Cell Lines Protocol - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Immunology/CellFreeze.html

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture. - [Read Freezing and Thawing of Mammalian Cell Lines Protocol]

Holzer's Stain Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/HOLZER.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols

An appropriate term for glial fibers is 'nerve glue', because they provide the internal support of the central nervous system. There are four types of glial cells: astrocytes, oligosendroglia,microglia, and ependymal cells. The glia fibers are stained with crystal violet which are resistant to the aniline-chloroform differentiating solution. - [Read Holzer's Stain Protocol]

Human Oesophageal Culture Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/28.htm#TOP

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

This procedure describes a method for establishing short-term explant cultures of oesophageal mucosa. Adverse effects produced by exposure to radiation or test compounds can be detected as an inhibition of cell outgrowth. - [Read Human Oesophageal Culture Protocol]

Human Oesophageal Culture Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/28.htm#TOP

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

Protocol describes a method for establishing short-term explant cultures of oesophageal mucosa. Adverse effects produced by exposure to radiation or test compounds can be detected as an inhibition of cell outgrowth. - [Read Human Oesophageal Culture Protocol]

Human Thyroid Culture Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/29.htm#TOP

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

This method enables the culturing of thyroid cells without loss of differentiation and medium change. It is potentially useful for the long-term study of drug effects on the thyroid gland. - [Read Human Thyroid Culture Protocol]

Long Term Lambda Phage Storage Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda6.html

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

No special treatment is required to prepare a lysate for the active collection. The following procedure should be used for long-term storage of lambda clones in the archival collections. The phage are diluted in media containing 7% DMSO and frozen at -80 degrees C. - [Read Long Term Lambda Phage Storage Protocol]

Long Term Storage of Bacterial Strains Protocol - http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid02.html

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40% glycerol. - [Read Long Term Storage of Bacterial Strains Protocol]

Long Term Storage of Yeast Stocks Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast2.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Yeast strains may be stored indefinitely at low temperatures (-80 degrees C). Two archiving methods are presented below. In Method A , the cells are grown on a plate, while in Method B the cells are grown in liquid culture. - [Read Long Term Storage of Yeast Stocks Protocol]

Long-Term Culture of Mouse Cortical Neurons as a Model for Neuronal Development, Aging, and Death - http://neuroscience.jhu.edu/martin9-23.pdf

Category: Neuroscience: Neuron Cell Culture Protocols

Neuronal Cell Culture PDF. Lesuisse and Martin. Wiley Periodicals 2002. - [Read Long-Term Culture of Mouse Cortical Neurons as a Model for Neuronal Development, Aging, and Death]

Long-Term Storage of Unfrozen Tetrahymena Cultures in Soybean Medium Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4472

Category: Tetrahymena Protozoa Protocols

Long-term storage of unfrozen Tetrahymena is convenient when a laboratory is not using the cells frequently; however, cells maintained under these conditions often take a couple of days of incubation in culture medium to recover. - [Read Long-Term Storage of Unfrozen Tetrahymena Cultures in Soybean Medium Protocol]

Long-Term Storage Storage of Unfrozen Tetrahymena Cultures in Maintenance Culture Medium Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4471

Category: Tetrahymena Protozoa Protocols

Protocol provides a method for long-term storage of Tetrahymena cultures using maintenance culture medium. - [Read Long-Term Storage Storage of Unfrozen Tetrahymena Cultures in Maintenance Culture Medium Protocol]

Maintenance of Probes in Bacteria Including Escherichia coli Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/bactstor.html

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

Plasmid (pUC series) containing genomic DNA fragments are maintained in E. coli strain DH5aTM. The E. coli cultures are routinely cultured at 37 C on Luria-Bertani (LB) agar on or in LB broth containing Ampicillin (30 µg/ml) or Carbenicillin (50 µg/ml broth, 100 µg/ml agar). E. coli strains are usually preserved in stab agar or glycerol for mid-term storage and lyophilized for long-term storage. - [Read Maintenance of Probes in Bacteria Including Escherichia coli Protocol]

Maintenance of Stocks of Marine Hypotrichs Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4469

Category: Laboratory Model Organisms Protocols

Protocol describes the preparation of stocks of marine hypotrichs for long-term storage. Marine euplotids are maintained by serial passage of tube stocks. - [Read Maintenance of Stocks of Marine Hypotrichs Protocol]

Neural Stem Cell Culture: Neurosphere Generation, Microscopical Analysis and Cryopreservation - http://www.natureprotocols.com/2006/08/25/neural_stem_cell_culture_neuro.php

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Describes the steps in detail to isolate and expand neural stem cells in the form of neurospheres from tissue dissections of the post-natal mouse brain. Procedures for the long term passage of neurospheres and the cryopreservation of neurospheres are also provided. In addition to the guidelines and tips for generating neurosphere cultures, we describe the method to prepare neurospheres for analysis by light microscopy. - [Read Neural Stem Cell Culture: Neurosphere Generation, Microscopical Analysis and Cryopreservation]

Short-Term Storage of Tetrahymena Cultures Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4470

Category: Tetrahymena Protozoa Protocols

A number of methods can be used for storage of unfrozen Tetrahymena cultures in the laboratory. Cells that are maintained using the short-term storage are described in this protocol. - [Read Short-Term Storage of Tetrahymena Cultures Protocol]

Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Living Mice - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4595

Category: Reporter Gene Assays: Luciferase Assay Protocols

Protocol describes a split luciferase complementation assay that can be used to repetitively and noninvasively study the interaction of proteins in small living animals. After the expression of the appropriate vectors has been checked in cell culture in vivo, studies can be performed either by implanting transiently transfected cells for short-term analysis (maximum of 7 days), or with tumor models grown from tumor cells stably expressing the complete reporter system. - [Read Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Living Mice]

Storage of Sera Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4279

Category: Antibody Protocols: Antibody Handling Protocols

This protocol describes the methods of storage for antibody-containing sera. Antibodies are resistant to a broad range of mildly denaturing conditions, so long-term storage is relatively easy. - [Read Storage of Sera Protocol]

Articles (1-6 of 6)

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Coupling Cysteine Containing Peptides to Carrier Protein for Immunization and Polyclonal Antibody

This protocol is used in the production of polyclonal antibodies that recognize a peptide sequence of interest.

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.