temperatures Protocols

Search results for: temperatures

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Dideoxy-mediated Sequencing of DNA Using Taq DNA Polymerase Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3803

Category: DNA Protocols: DNA Sequencing Protocols: Dideoxy Mediated Sequencing Protocols

Because sequencing reactions catalyzed by thermostable DNA polymerasessuch as Taq are carried out at elevated temperatures, problemscaused by mismatched annealing of primers or templates richin secondary structure are greatly alleviated. - [Read Dideoxy-mediated Sequencing of DNA Using Taq DNA Polymerase Protocol]

Long Term Storage of Bacterial Strains Protocol - http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid02.html

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40% glycerol. - [Read Long Term Storage of Bacterial Strains Protocol]

Long Term Storage of Yeast Stocks Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast2.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Yeast strains may be stored indefinitely at low temperatures (-80 degrees C). Two archiving methods are presented below. In Method A , the cells are grown on a plate, while in Method B the cells are grown in liquid culture. - [Read Long Term Storage of Yeast Stocks Protocol]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

A Great PCR and Multiplex Guide. Topics for PCR optimization. Primer conditions, PCR temperatures, etc. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: PCR Protocols: PCR Optimization

A Great PCR and Multiplex Guide. Topics for PCR optimization. Primer conditions, PCR temperatures, etc. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR Genotyping from Tail DNA Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/P4.html

Category: Genetics and Genomics: Genotyping Protocols: PCR Genotyping

Protocol for PCR genotyping from tail DNA. This protocol works well for a variety of genes and primer pairs including Tg and KO alleles. Oligonucleotide melting temperatures between 60° and 65° seem to work well. - [Read PCR Genotyping from Tail DNA Protocol]

Reagents and Guidelines for Long PCR - http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Long PCR Protocols

Long PCR Buffer, Cycle times and temperatures, Picking Primers, Hot Start Polymerases for Long PCR. Harvard-Lipper Center for Computational Genetics. Modified from Cheng et al. - [Read Reagents and Guidelines for Long PCR]

Articles (1-2 of 2)

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

[1-2]