PCR cycle steps, protocol using SuperScript II reverse transcriptase (Life Technologies) and Taq polymerase, Gene specific primer, T17 Adapter primer and an Adapter primer. Dr.Dawson, Nottingham. - [Read 3' Rapid Amplification of cDNA Ends (RACE) Protocol]
AFLP® technology was developed initially by Keygene in the early 1990’s. AFLP has become a widespread genetic fingerprinting technology.
AFLP video and diagrams. Keygene - [Read AFLP - Genetic Fingerprinting]
The role of microspheres in these screens is similar to their
traditional role in immunoassays, namely as a solid phase to
either enhance detection, separation, or both. The predominance of radioactive assays in high-throughput screening, along with the desire to find alternative means of detection, have led to research on substituting alternative fluorescent technologies. - [Read Microspheres for High-Throughput Screening Assays]
The Handbook — A Guide to Fluorescent Probes and Labeling Technologies is a comprehensive resource for fluorescence technology and its applications. Newly revised, The Handbook contains detailed information describing the use of more than 3000 Molecular - [Read Molecular Probes Handbook Invitrogen]
Using molecular marker technology in studies on plant genetic diversity. DNA-based technologies: PCR-based technologies
Amplified fragment length polymorphisms (AFLPs. Includes: AFLP technology, step by step; DNA digestion and ligation; PCRs and detection; Summarising the technology. - [Read PCR-Based Technologies Amplified Fragment Length Polymorphisms (AFLPs)]
Protocol is the second in a set of three, describing fluorescent mRNA differential display (FDD or FDDRT-PCR). For the purposes of FDD gene expression analysis, as well as any other RNA-based gene expression technologies, contaminating genomic DNA must be removed before reverse transcription and subsequent PCR. - [Read Removal of Genomic DNA from Total RNA for Use in Fluorescent mRNA Differential Display Protocol]
Zero Blunt TOPO PCR Cloning Kit, Detailed protocol.
(Invitrogen Life technologies). Alam Lab. Department of Microbiology, University of Hawai'i. - [Read Zero Blunt TOPO PCR Cloning Kit Protocol]