techniques Protocols

Category: Molecular Biology Protocols: Agarose Gel Preparation

Solutions for Agarose gel prepartion, Selection of Appropriate Gel Size and Composition. Mycology Lab University of Tennessee - [Read Agarose Gel Preparation Protocol]

Category: Cell Biology and Cell Culture: Cell Culture Techniques

ECACC Handbook of Cell Culture. Fundamental Techniques in Cell Culture: A Labratory Handbook. Sigma Aldrich. Excellent Guide to Cell Culture, aseptic technique, storage of cell lines, subculture of cells. - [Read Handbook of Cell Culture. - Sigma Aldrich.]

Category: PCR Protocols: Standard PCR Protocol

Standard PCR Protocol - Molecular Biology Techniques Manual - Rybicki - [Read Standard PCR Protocol - Molecular Biology Techniques Manual]

Category: PCR Protocols: Standard PCR Protocol

Calculating Concentrations for PCR - Molecular Biology Techniques Manual - Rybicki - [Read Calculating Concentrations for PCR]

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Protocols for cell fixation and tissue fixation. HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block techniques. Embedding Tissue Into Frozen Blocks. Fixing Cells with Fresh Paraformaldehyde. Preparation of Frozen Sections. Immunohistochemistry. Paraffin sections and Frozen sections. DERC Univ. Colorado PDF. - [Read HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block PDF]

Category: RNA Protocols: RT-PCR and cDNA synthesis

REVERSE TRANSCRIPTION PCR: Molecular Biology Techniques Manual - Rybicki - [Read REVERSE TRANSCRIPTION PCR: Molecular Biology Techniques Manual]

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Cultured mammalian cells are used extensively in cell biology studies; it requires a number of special skills in order to be able to preserve the structure, function, behavior and biology of the cells. This unit describes the basic skills required to maintain and preserve cell cultures: aseptic technique, medium characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. - [Read Basic Techniques for Mammalian Cell Tissue Culture Protocol]

Category: PCR Protocols: PCR Optimization

PCR PRIMER DESIGN AND REACTION OPTIMISATION: Molecular Biology Techniques Manual - Rybicki - [Read PCR PRIMER DESIGN AND REACTION OPTIMISATION: Molecular Biology Techniques Manual]

Category: Plant Biology Protocols: Plant Tissue Culture

Plant tissue culture techniques. Pages 151-174, in Tested studies for laboratory ... engineering protocols employ callus initiation procedures after DNA has ... Mineo Zoology Univ. Toronto - [Read Plant Tissue Culture Techniques]

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

FISH Guide and Troubleshooting. Includes: Cytogenetic techniques; Slide preparation; When do chromosomes spread; Cell suspensions; G-banding; Cell fixatives; Slide preparation factors. - [Read FISH Guide and Troubleshooting]

Category: Protein Protocols: Protein Expression Protocols

Protein Expression Techniques Advantages and Disadvantages. Molecular Station. - [Read Protein Expression Techniques Advantages and Disadvantages]

Category: PCR Protocols: Standard PCR Protocol

"CORE SAMPLE" PCR - A method to re-PCR unique bands from products of mixed size - Molecular Biology Techniques Manual - Rybicki - [Read "CORE SAMPLE" PCR - A method to re-PCR unique bands from products of mixed size]

Category: Cell Culture Protocols: Sterile Technique Protocols

Cultured mammalian cells are used extensively in cell biology studies; it requires a number of special skills in order to be able to preserve the structure, function, behavior and biology of the cells. This unit describes the basic skills required to maintain and preserve cell cultures: aseptic technique, medium characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. - [Read Basic Techniques for Mammalian Cell Tissue Culture Protocol]

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Materials for cresyl violet staining protocol (see methods section) ... Histology also refers to the techniques used to prepare tissue for microscopic study ... Neuroscience Histology Methods - [Read Neuroscience Histology Methods]

Category: Electrophysiology Ion Channels

Electrophysiology - The Patch Clamp Technique. Pictures and description. Assmann - [Read Electrophysiology - The Patch Clamp Technique]

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Cryopreserved PBMCs are a common specimen source for studies of immunological responses to vaccines, immunotherapies, etc. The health and viability of cells recovered post-cryopreservation are of course critical to the success and accuracy of immunological assays performed on them. This protocol standardizes PBMC isolation and cryopreservation techniques, specifically for the assessment of thawed cells by cytokine flow cytometry. - [Read Protocol for Isolation, Cryopreservation, and Thawing of PBMCs]

Category: Cell Biology and Cell Culture

Fundamental Techniques in Cell Culture: A Handbook. Excellent Resource. Sigma. - [Read Fundamental Techniques in Cell Culture: A Handbook]

Category: Immunology: Antigen Retrieval

Antigen Retrieval. Principles and Technique. A handbook of Immunochemical Staining Methods and techniques. Marc Key - [Read Antigen Retrieval PDF]

Category: Mass Spectrometry Protocols

Peptide Mapping Analysis Techniques. Dr.Aebersold, Vrije Universiteit Amsterdam - [Read In-gel digestion of proteins for peptide fingerprint mapping]

Category: Immunology: Mononuclear Cell Isolation Protocols

Covers techniques for preparing lymphoid cell suspensions and removing red blood cells and dead cells. - [Read Isolation of Mouse Mononuclear Cells Protocol]

Category: Transfection Protocols: Stable Transfection Protocols

Pulsed electrical fields can be used to introduce DNA into a wide variety of animal cells. Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate-DNA coprecipitation. But, as with other transfection methods, the optimal conditions for electroporating DNA into untested cell lines must be determined experimentally. - [Read DNA Transfection by Electroporation]

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

Includes Abbreviations, Background, and Procedure steps using BSA. The Bradford protein assay (1) is one of several simple methods commonly used to determine the total protein concentration of a sample.  The method is based on the proportional binding of the dye Coomassie to proteins. The assay is colorimetric; as the protein concentration increases, the color of the test sample becomes darker.  Coomassie absorbs at 595 nm.  - [Read Bradford Protein Concentration Assay]

Category: Immunology: Chemotaxis

Chemotaxis Laboratory Lab, Introduction to Dictyostelium and Chemotaxis. - [Read Chemotaxis Laboratory Lab]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

Calculating Concentrations for PCR - Molecular Biology Techniques Manual - Rybicki - [Read Calculating Concentrations for PCR]

Category: Immunology: Chemotaxis

Chemotaxis Protocol by Jeff Hadwiger. Dictyostelium Chemotaxis to Folate: a Simple Assay, from the Hadwiger Laboratory. Detailed Protocol. - [Read Chemotaxis Protocol]