User Panel

My Panel

Bookmark Science Articles

technical Protocols

Search results for: technical

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography - http://www.glycotech.com/protocols/Proto6.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

High performance liquid affinity chromatography (HPLAC) is a useful procedure to investigate he interactions between carbohydrate binding protein and their ligands. Technical requirements are similar to conventional HPLC. HPLAC can screen and separate natural ligands from complex biological mixtures. WeiTong Wang~GlycoTech Corporation, Rockville, Maryland - [Read Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography]

Analysis of Proteins by Immunoprecipitation - http://dps.ufl.edu/hansen/protocols/imp98.prt.htm

Category: Protein Protocols: Immunoprecipitation Protocols

Antibody-antigen complexes are removed from solution by addition of an insoluble form of an antibody binding protein such as Protein A, Protein G or second antibody. Immunoprecipitation protocols / methodology and technical background information. P.J. Ha - [Read Analysis of Proteins by Immunoprecipitation]

Basic Ideas in PCR Protocol Design. PDF - http://www.idtdna.com/support/technical/TechnicalBulletinPDF/A_Basic_PCR_Protocol.pdf

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Basic Ideas in PCR Protocol Design. PDF - [Read Basic Ideas in PCR Protocol Design. PDF]

Basic Ideas in PCR Protocol Design. PDF - http://www.idtdna.com/support/technical/TechnicalBulletinPDF/A_Basic_PCR_Protocol.pdf

Category: PCR Protocols

Basic Ideas in PCR Protocol Design. PDF - [Read Basic Ideas in PCR Protocol Design. PDF]

Blastocyst Embryo Transfer into Pseudopregnant Mouse Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp6.html

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol for blastocyst embryo transfer into pseudopregnant recipient female mouse. - [Read Blastocyst Embryo Transfer into Pseudopregnant Mouse Protocol]

Breeding Mice Protocol - http://members.cox.net/microinjectionworkshop/technical/breeding.html

Category: Mouse Protocols: Mouse Embryology Protocols

Information and protocol for breeding mice. - [Read Breeding Mice Protocol]

Detection of Estrus in Mice - http://members.cox.net/microinjectionworkshop/technical/estrus.html

Category: Mouse Protocols: Mouse Embryology Protocols

Information on the detection of estrus in mice. - [Read Detection of Estrus in Mice]

ELISA Procedure for Measuring Serum Antibody Titer - http://www.komabiotech.com/technical/protocol/ELISA.htm

Category: ELISA Protocols

Method and ELISA Procedure for Measuring Serum Antibody Titer. Komabiotech - [Read ELISA Procedure for Measuring Serum Antibody Titer]

ES / MEF Cell Culture and Electroporation of Targeting Construct Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp5.html

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Protocol for ES/MEF cell culture and electroporation of targeting construct. - [Read ES / MEF Cell Culture and Electroporation of Targeting Construct Protocol]

ES / MEF Cell Culture and Electroporation of Targeting Construct Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp5.html

Category: Stem Cell Protocols: Mouse Embryonic Fibroblasts Protocols

Protocol for ES/MEF cell culture and electroporation of targeting construct. - [Read ES / MEF Cell Culture and Electroporation of Targeting Construct Protocol]

ES / MEF Cell Culture and Electroporation of Targeting Construct Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp5.html

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Protocol for ES / MEF cell culture and electroporation of targeting construct. - [Read ES / MEF Cell Culture and Electroporation of Targeting Construct Protocol]

ES / MEF Cell Culture and Electroporation of Targeting Construct Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp5.html

Category: Mouse Protocols: Mouse Cell Culture Protocols

Protocol for ES / MEF cell culture and electroporation of targeting construct. - [Read ES / MEF Cell Culture and Electroporation of Targeting Construct Protocol]

Mouse Embryo Transfer into Pseudopregnant Recipient Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp2.html

Category: Mouse Protocols: Mouse Embryology Protocols

1-cell embryo transfer into pseudopregnant recipient female mouse. - [Read Mouse Embryo Transfer into Pseudopregnant Recipient Protocol]

Natural Pathogens of Laboratory Mice and Rats - http://members.cox.net/microinjectionworkshop/technical/pathogen.html

Category: Mouse Protocols

For all the transgenic or other mouse researchers out there who have had to explain why colony infections are not just bad for the welfare of the animals concerned, but may invalidate the ongoing studies as well. - [Read Natural Pathogens of Laboratory Mice and Rats]

Overview of Flow Cytometry - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661BFDC3F5C6C63380B3829E2D6599&objectid=6674E52FF6A6D9943372F453033D787C

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Provides an overview of terminology and echniques unique to flow ytometry and is divided into two sections. The first section discusses technical aspects of flow cytometry which apply primarily to the instrumentation-oriented flow cytometry phase. The second section discusses electronic cell separation using flow cytometry. - [Read Overview of Flow Cytometry]

PCR of Blood, Hair or Small Tissue Samples of Mouse Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp8.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for the PCR of blood, hair or small tissue samples of mouse. Includes: Preparation of blood or a tissue sample obtained by ear punch for PCR; Preparation of hair shafts for PCR. - [Read PCR of Blood, Hair or Small Tissue Samples of Mouse Protocol]

Planning an Effective Mouse Colony - http://members.cox.net/microinjectionworkshop/technical/tgcolny1.html

Category: Mouse Protocols: Mouse Embryology Protocols

Considerations in planning an effective mouse colony for transgenic production. Includes: Mouse strain; Colony size; Efficiency of superovulation; Parental Suitability; Pseudo-pregnancy. - [Read Planning an Effective Mouse Colony]

Tail DNA Preps for Genomic Southerns Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp10.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for tail DNA preps for genomic southerns. - [Read Tail DNA Preps for Genomic Southerns Protocol]

Technical information on 2-D PAGE - http://us.expasy.org/ch2d/protocols/

Category: Proteomic Protocols: 2-D Gel Electrophoresis

Very Concise Protocol for 2D Electrophoresis. ExPASY. - [Read Technical information on 2-D PAGE]

Vasectomy of Mouse Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp9.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for the vasectomy of mouse. Includes: The Anesthetic; Test Breeding. - [Read Vasectomy of Mouse Protocol]