Protocol for the dissection of Drosophila CNSs. Includes: Tools; Dissection of the CNS; Dissecting larval CNSs; Dissecting early pupae; Dissecting mid pupae; Dissecting late pupae; Dissecting the whole CNS; Dissecting only the brain proper; Taking the pupa out of the puparium. - [Read Dissection of Drosophila CNSs Protocol]
This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.