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5'-End cDNA Amplification Using Classic RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4131

Category: PCR Protocols: cDNA Synthesis Protocols

To generate "5'-end" partial cDNA clones using classic RACE, the first-strand products are generated by reverse transcription (primer extension) from a known gene-specific primer (GSP-RT). Then, a poly(A) tail is appended using terminal deoxynucleotidyltransferase (Tdt) and dATP. Amplification is carried out using three primers. - [Read 5'-End cDNA Amplification Using Classic RACE Protocol]

Amplification of Insert End Sequences from BACs Clones by Thermal Asymmetric Interlaced PCR - http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb16/16175-2.pdf

Category: BACs Bacterial Artificial Chromosome Protocols

Protocol presents the amplification of insert end sequences from bacterial artificial chromosome clones using TAIL-PCR. The amplified products are suitable as probes for chromosome walking and genome mapping and as templates for direct sequencing. The protocol has been used in rice genome studies. - [Read Amplification of Insert End Sequences from BACs Clones by Thermal Asymmetric Interlaced PCR]

Blood Collection by Tail Bleeding Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4388

Category: Mouse Protocols: Mouse Surgical Procedures Protocols

Protocol describes a method for collection of blood from the tail of a mouse. The blood can then be used for analysis. - [Read Blood Collection by Tail Bleeding Protocol]

Cytogenetic Protocol Blood Preps - http://www.jax.org/cyto/blood_preps.html

Category: Cytogenetics Protocols

Cytogenetic protocol for blood preps. Includes: Preparation of materials; Culture Procedure - Use aseptic technique throughout procedure until harvest; Harvest; Slide making; Collecting blood by tail vein method; PHA Dose Response. - [Read Cytogenetic Protocol Blood Preps]

Differential Display-PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3844

Category: PCR Protocols

Method uses PCR to amplify and display many cDNAs derived from the mRNAs of a given cell or tissue type. The method relies on two different types of synthetic oligonucleotides: anchored antisense primers and arbitrary sense primers. A typical anchored primer is complementary to approx. 13 nucleotides of the poly(A) tail of mRNA and the adjacent two nucleotides of the transcribed sequence. - [Read Differential Display-PCR Protocol]

DNA from Tail Biopsies - http://www.jax.org/imr/tail_phenol.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

DNA from Tail Biopsies, Mouse tail clipping DNA genotyping. Jackson Lab. - [Read DNA from Tail Biopsies]

Genotyping Protocol: T1320 mouse - http://www.mmrrc.org/strains/425/ctr_protocol.pdf

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

Genotyping Protocol: MMRRC 425. Assay Type: PCR - can distinguish heterozygous animals from homozygous animals. DNA Extraction: DNA from tail snips was ... - [Read Genotyping Protocol: T1320 mouse]

Hydrodynamics-Based Gene Transduction Protocol - http://www.natureprotocols.com/2006/06/29/hydrodynamicsbased_gene_transd.php

Category: Gene Delivery Protocols

This highly efficient in vivo gene transduction technique for laboratory mice. Hepatocytes are most effectively transduced by tail vein injection of a large volume of DNA solution in a short time. Practice with the injection technique is necessary!!! - [Read Hydrodynamics-Based Gene Transduction Protocol]

Isolation of DNA from Mouse Tail Biopsies - http://www.med.umich.edu/tamc/tDNA.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

Isolation of DNA from Mouse Tail Biopsies. University of Michigan Transgenic Animal Model Core, Transgenic Animal Web. - [Read Isolation of DNA from Mouse Tail Biopsies]

Lac-Z Detection in Tail Biopsies - http://www.jax.org/imr/tgr26_staining.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

ROSA26 : Lac-Z Detection in Tail Biopsies, the Jackson Laboratory. - [Read Lac-Z Detection in Tail Biopsies]

Mouse Tail DNA Extraction Protocol - http://www.ohsu.edu/nsi/faculty/reddyh/lab/protmsdn.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for mouse tail DNA extraction. - [Read Mouse Tail DNA Extraction Protocol]

PCR Genotyping from Tail DNA Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/P4.html

Category: Genetics and Genomics: Genotyping Protocols: PCR Genotyping

Protocol for PCR genotyping from tail DNA. This protocol works well for a variety of genes and primer pairs including Tg and KO alleles. Oligonucleotide melting temperatures between 60° and 65° seem to work well. - [Read PCR Genotyping from Tail DNA Protocol]

Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4038

Category: Mouse Protocols: Mouse Genetics Protocols

Method is used chiefly to genotype transgenic and knockout mice. Each 6-10-mm snippet of mouse tail yields 50-100 µg of DNA that can be used in dot or slot blotting to detect a transgene of interest, in Southern hybridization to detect DNA fragments that are <20 kb in size, and as a template in PCRs. - [Read Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol]

Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4038

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

Simple protocol is used to extract DNA from small numbers of cultured cells and from fragments of soft or bony tissues. The method is used chiefly to genotype transgenic and knockout mice. Each 6-10-mm snippet of mouse tail yields 50-100 µg of DNA that can be used in dot or slot blotting to detect a transgene of interest, in Southern hybridization to detect DNA fragments that are <20 kb in size, and as a template in PCRs. - [Read Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol]

Preparation of Mouse Tail DNA for Dot Blots or PCR - http://www.muridae.com/wmc/docs/tailDNA_prep.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

Tail dots blot, quicker than doing southerns. Muridae.com, Eric Mercer - [Read Preparation of Mouse Tail DNA for Dot Blots or PCR]

reparation of Copy Standards for Southern Blot Copy Number Determination - http://www.med.umich.edu/tamc/spike.html

Category: Transgenic Mouse Protocols: Mouse Genotyping Protocols

PCR screens must be designed to detect transgene DNA at the single copy level.Copy standards are prepared by mixing non-transgenic tail DNA with a known amount of transgene DNA to produce transgene copy standards. University of Michigan Transgenic Animal - [Read reparation of Copy Standards for Southern Blot Copy Number Determination]

Tail DNA for PCR (No Organic Solvents) - http://www.jax.org/imr/tail_nonorg.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

Use mouse tail DNA directly for PCR after Proteinase K digestion. The Jackson Lab. - [Read Tail DNA for PCR (No Organic Solvents)]

Tail DNA Preparation Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/TailDNA.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Isolation from Mammalian Cells Protocols

Protocol for Tail DNA preparation. - [Read Tail DNA Preparation Protocol]

Tail DNA Preps for Genomic Southerns Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp10.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for tail DNA preps for genomic southerns. - [Read Tail DNA Preps for Genomic Southerns Protocol]

TAIL PCR Protocol - http://preuss.bsd.uchicago.edu/protocols/tail.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

TAIL PCR Protocol. TAIL is a series of reactions that are intended to map where a T-DNA (transfer DNA) has inserted within the genome. The main components of the 3 reactions are the AD (Arbitrary Degenerate) primers, border primers, and DNA from the T-DNA - [Read TAIL PCR Protocol]

TAIL PCR Protocol - http://preuss.bsd.uchicago.edu/protocols/tail.html

Category: PCR Protocols

Articles (1-5 of 5)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Constructing Random-Peptide Libraries for Phage Display

The protocol describes the construction of a large (10^8 to 10^10) primary random peptide phage library in fUSE5 or f88-4 strains.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

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