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Labeling Antibodies with Iodine Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4287

Category: Antibody Protocols: Antibody Labeling Protocols

This protocol describes a simple chemical oxidation method for labeling antibodies with iodine. Iodide-125 (supplied as NaI) is oxidized to form iodine-125 (I2), which attacks tyrosyl and histidyl side chains. The iodinated antibodies are easily detected and quantitated using gamma counters or film. They are used primarily in immunoassays, but other techniques can be adapted conveniently to the iodine detection method. - [Read Labeling Antibodies with Iodine Protocol]

Storage of Purified Antibodies - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4282

Category: Antibody Protocols: Antibody Handling Protocols

This protocol describes the storage of "homemade" purified antibodies. Antibodies from commercial sources are normally supplied with proper storage conditions. - [Read Storage of Purified Antibodies]