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First strand cDNA synthesis with Super Script II Protocol - http://www.methods.info/Methods/cDNA/SSII.html

Category: PCR Protocols: cDNA Synthesis Protocols

This method of first strand cDNA synthesis is more efficient than Ready-to-Go beads. You can use as little as 100ng total RNA and still detect you gene expression in PCR. - [Read First strand cDNA synthesis with Super Script II Protocol]

Gel Super-Shift Assay EMSA Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/III.%20Proteins/C.%20DNA-Protein%20Interactions/3b.%20Super-shift.pdf

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Protocol for gel super shift assay EMSA. - [Read Gel Super-Shift Assay EMSA Protocol]

Protocol for Motility using VE-DIC Microscopy - http://www.proweb.org/kinesin/Methods/VE-DIC.html

Category: Cell Biology and Cell Culture

Protocol for motility using VE-DIC microscopy. Materials required: Anti-GST (glutathione S-transferase) antibody, PB buffer =10 mM NaPO4 pH 7.2, EGTA, MgCl2, Clarified motor lysate, MTs or Axoneme-MTs,MgÂ·ATP, VALAP (1 Vasoline: 1 Lanolin: 1 Paraffin, heated gently until melted) and Maxell XR-S120 Black Magnetite Super-VHS tapes or comparable. - [Read Protocol for Motility using VE-DIC Microscopy]

Articles (1-10 of 11)

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.