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Agarose Overlay Assay Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/31.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

Protocol descibes the use of L929 mouse fibroblast cells cultured in vitro in an agarose overlay assay to assess the toxicity of test substances. The assay may be useful in assessing the irritation potential of test substances (e.g. surfactant-based products) as an alternative to the Draize rabbit eye test. - [Read Agarose Overlay Assay Protocol]

Hamster Ovary Cell NA+/K+ -ATPase Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/35.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

Accumulation of lipophilic substances in the plasma membrane may affect the membrane lipid order and consequently affect the function of these proteins. Changes in the activity of the Na+/K+ -ATPase, which is the major active transport system responsible for the electrochemical potential in mammalian cells, can therefore be an indication of the effect that a chemical may have on the viability of the cell membrane and possibly the whole cell. - [Read Hamster Ovary Cell NA+/K+ -ATPase Test]

Ovary Cell Proliferation Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/36.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

The rate of cellular proliferation may be regarded as an overall indicator of the physiological status of the cell. Therefore, the effect of various toxic substances on different cell functions will be reflected by changes in the proliferation rate. - [Read Ovary Cell Proliferation Test]

Principles of Anesthesia and Analgesia: ESSENTIALS FOR ANIMAL RESEARCH - http://research.ucsb.edu/connect/acc/esanesth.htm

Category: Animal Research Techniques

Legal responsibilities, Recognition and treatment of pain, Anesthetic monitoring, stages and planes of general anesthesia, injecting, inhalation, species-specific, anesthetic emergencies, controlled substances. Marilyn J. Brown UCSB - [Read Principles of Anesthesia and Analgesia: ESSENTIALS FOR ANIMAL RESEARCH]

Yeast Growth Rate Cytotoxicity Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/33.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Microorganism Cytotoxicity Assays Protocols

The proliferation rate of the yeast, Saccharomyces cerevisiae, may be regarded as an overall indicator of the physiological status of the cell. Therefore, the effect of various toxic substances on different cell functions will be reflected by changes in the rate of proliferation. It is possible to determine the toxicity of a test substance simply by measuring cell density. - [Read Yeast Growth Rate Cytotoxicity Test]

Yeast Plasma Membrane H+ -ATPASE Toxicity Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/34.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Microorganism Cytotoxicity Assays Protocols

Accumulation of lipophilic substances, many of which may be environmental chemicals, affects the membrane lipid order and consequently affects the functions of these proteins. Since, the function of important cellular proteins, such as the H+-ATPase strongly depends upon the integrity of the lipid bilayer, the activity of the H+-ATPase may be used as a sensitive indicator of the effect that a chemical may have on the viability of the cell. - [Read Yeast Plasma Membrane H+ -ATPASE Toxicity Test]