sterilization Protocols

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Formaldehyde Treatment of Tissue Culture Hoods - http://www.nottingham.ac.uk/~mbzspd/methods/Formaldehyde_Treatme.html

Category: General Research Protocols and Methods: Sterile Technique

Formaldehyde sterilization of tissue culture hoods. A bit toxic however effective. Dr.Dawson, Department Biochemistry, Univ. Nottingham Medical School. - [Read Formaldehyde Treatment of Tissue Culture Hoods]

Plant Cell Purification of Intact Plant Protoplasts - http://www.axis-shield.com/densityhome/optiprep/C18.pdf

Category: Cell Biology and Cell Culture

Cell fractionation protocol to yield intact plant protoplasts. Technique used to purify protoplasts from the grass Glyceria fluitans. Protocol includes: Determination of leaf osmolality; Sterilization. - [Read Plant Cell Purification of Intact Plant Protoplasts]

Principles of Aseptic Technique: ESSENTIALS FOR ANIMAL RESEARCH - http://research.ucsb.edu/connect/acc/esasept.htm

Category: Animal Research Techniques

Facilities, equipment, sterilization, steam, gas, liquid, animal research, preparation of the animal, surgical pack, surgeon preparation. - [Read Principles of Aseptic Technique: ESSENTIALS FOR ANIMAL RESEARCH]

Soybean Cotyledonary-node Agrobacterium-mediated Transformation System - http://www.biotech.unl.edu/transgenic/protocols.html

Category: Plant Biology Protocols

Protocol includes: Seed Sterilization Step; Seed Germination Step; Agrobacterium Inoculum Preparation; Explant Preparation; Co-cultivation Plates; Shoot Initiation Step; Shoot Elongation Step; Rooting Step. - [Read Soybean Cotyledonary-node Agrobacterium-mediated Transformation System]

Sterilization and Filtration Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B9D0482A943EC97AC4807854493&objectid=6673A220D50910E56F81295AE515690F

Category: Cell Culture Protocols: Sterile Technique Protocols

This unit on sterility in the tissue culture environment describes methods for sterilization of liquid and dry goods used for tissue culture and filtration of liquids to prevent contamination of cultures. - [Read Sterilization and Filtration Protocol]

Sterilization of Plant Culture Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Media_Sterilization.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for sterilization of plant culture media. Includes: Minimum Autoclaving Time for Plant Tissue Culture Medium. - [Read Sterilization of Plant Culture Media Protocol]

Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_220-228.pdf

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol for whole mount fluorescence in situ hybridization (FISH) of repetitive DNA sequences on interphase nuclei of the small cruciferous plant Arabidopsis thaliana. Includes: Seed sterilization and germination; Tissue fixation; Labeling of the probe DNA; Pretreatment; In situ hybridization; Pre-absorption of antibodies; Posthybridization washes; Immunocytochemical detection; Direct detection; Indirect detection; Staining and mounting; Fluorescence microscopy. - [Read Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase]