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Simplifying hESC culture - http://www.bloodjournal.org/cgi/content/full/105/12/4550

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

Human embryonic stem cells are a valuable resource for research and cell replacement therapy but are notoriously cumbersome to culture. Bhatia and colleagues show that an increased dose of basic fibroblast growth factor eliminates the need for feeder laye - [Read Simplifying hESC culture]

Stem Cell RT-PCR Protocol - http://stemcells.nih.gov/research/NIHresearch/scunit/RTPCR.asp

Category: RNA Protocols: RT-PCR and cDNA synthesis

Stem Cell RT-PCR Protocol. NIH Stem Cell Division - [Read Stem Cell RT-PCR Protocol]

Subcloning Protocol ES Cells - http://stemcells.nih.gov/research/NIHresearch/scunit/subcloning.asp

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Subcloning Protocol ES Cells. Used an adapted form of the feeder-free protocols detailed in Xu et al. (Nature Biotechnology 19:971â€“974, 2001). NIH Stem Cell Unit - [Read Subcloning Protocol ES Cells]

Subcutaneous Injection of Embryonic Stem (ES) Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4383

Category: Stem Cell Protocols: Stem Cell Injection Protocols

This protocol describes a method for subcutaneous injection of ES cells into mice. - [Read Subcutaneous Injection of Embryonic Stem (ES) Cells Protocol]

Vegetative Propagation of Alfalfa by Stem Cuttings - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/AlfalfaVegetativePropagationByStemCuttings.pdf

Category: Plant Biology Protocols

Alfalfa is an outcrossing species, cultivars consist of populations rather than individual homozygous or inbred lines. After only two cycles of self-pollination, severe inbreeding depression eliminates selfed individuals from populations. To obtain sufficient plant material of relative genetically uniformity (these plants will still necessarily be heterozygous) for experimental purposes, it may be necessary to propagate a single individual or clone. - [Read Vegetative Propagation of Alfalfa by Stem Cuttings]

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Microinjection Pipettes Protocol

Microinjection Pipettes Preparation Protocol.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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