stanford Protocols

Category: Proteomic Protocols: 2-D Gel Electrophoresis

2x Lysis Buffer Recipe. Utz Lab. - [Read 2x Lysis Buffer Recipe]

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for 96-well confirmation Yeast PCR. Includes: Clonal purification; Generate a master plate (96-well format); Making a frozen backup stock; Confirmation PCR for one Row; ORF Specific Confirmation PCR --> "A-B" primers (upstream junction); Transfer template DNA to multiwell PCR plate; Prepare and dispense master mix for A-B PCR. - [Read 96-Well Confirmation Yeast PCR Protocol]

Category: Protein Protocols: Antibody Purification Protocols

Affinity Purification of Antibodies. Vesicle Trafficking. - [Read Affinity Purification of Antibodies]

Category: Plant Biology Protocols: Plant Genetics Protocols

AFLP was designed as a highly sensitive method for DNA fingerprinting to be used in a variety of fields. We are using this technology to generate DNA based markers for cloning genes involved in phototropic responses in higher plants that have only been identified genetically by mutant phenotype. Protocol includes: Generate polymorphic recombinant F2 (or F3) population; Isolate genomic DNA; Restriction of DNA; Ligation of adapters; Pre-amplification of template DNA; AFLP-PCR; etc. - [Read AFLP For Positional Cloning]

Category: PCR Protocols: AFLP PCR

Mannie Liscum and Paul Oeller. Department of Plant Biology. Carnegie Institution of Washington, Stanford. AFLP technology is used here to generate DNA based markers for cloning genes involved in phototropic responses in higher plants that have only been i - [Read AFLP: not only for fingerprinting, but for positional cloning]

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for an alternative method for sporulation for BY4743. - [Read Alternate Sporulation Method for BY4743 Protocol]

Category: Antibody Microarray Protocols

Antibody Array Production and Data Analysis. Probing Antigen Arrays and washing/ Blocking. Great Protocols and information. Brian Kidd. Stanford. - [Read Antibody Array Production and Analysis]

Category: Microarray Protocols: Microarray Analysis Protocols

Protocol for aRNA labeling and hybridization. - [Read aRNA Labeling and Hybridization Protocol]

Category: Cytogenetics Protocols: Array CGH

Array CGH Protocol. Stanford. protocol is an amalgamation of protocols. - [Read Array CGH Protocol]

Category: RNA Protocols: cDNA Libraries Library

Methods and procedure for Colony Hybridization. Vesicle Trafficking Home. - [Read Colony Hybridization Protocol]

Category: DNA Protocols: DNA Sequencing Protocols

Protocol first describes the vector preparation and, then, describes the insert preparation.  Vital to have an excellent vector in order to produce a sequencing library.  Protocol employs the male-specific coliphage M13 as the sequencing vector.  M13 is a filamentous phage with a single-stranded, circular genome.  M13 is widely used as a vector because many versions are available commercially and because M13 has certain advantages. - [Read Construction of the Sequencing Library Protocol]

Category: DNA Microarray Protocols

Cyanine Dye Purification Protocol using Sep-Pak and HPLC purification protocol for Cy dyes (un)incorporated with dNTP. Brown Lab Howard Hughes Medical Institute - [Read Cyanine Dye Purification Protocol Cy]

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Fugene Transfection Protocol PDF. Meyer Lab Standford. - [Read Fugene Transfection Protocol PDF]

Category: Microarray Protocols: Microarray DNA Labeling Protocols

Protocol for genomic DNA labeling. - [Read Genomic DNA Labeling Protocol]

Category: Nucleic Acid Modification Protocols: Nucleic Acid Labeling Protocols

Protocol for genomic DNA labeling. - [Read Genomic DNA Labeling Protocol II]

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

The procedures involve the isolation and growth of primary cell cultures from rodent and human tissue as well as the use of viral vectors for the introduction and expression of mammalian genes in cells in culture and in live rodents. - [Read Growth of Primary Cell Culture and Viral Vector Handling Protocols]

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Detailed protocols and methods for GST protein purification. Vesicle Trafficking, Stanford. - [Read GST Fusion Protein Prep]

Category: DNA Microarray Protocols

Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation Washing and Scanning Arrays. Brown Lab. - [Read Human DNA Microarray Hybridization]

Category: DNA Microarray Protocols

Hybridization of Arrays. . General/Yeast Microarrays. Brown Lab - [Read Hybridization of Arrays]

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for immunohistochemistry on Drosophila brains. - [Read Immunohistochemistry on Drosophila Brains Protocol]

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for in situ hydbridization to tissue sections. Includes: Paraffin Sections; Frozen Sections; Probe preparation; Hybridization and washes; Autoradiography. - [Read In Situ Hybridization to Tissue Sections Protocol]

Category: Mouse Protocols: Mouse Cell Culture Protocols

The procedures involve the isolation and growth of primary cell cultures from rodent and human tissue as well as the use of viral vectors for the introduction and expression of mammalian genes in cells in culture and in live rodents. - [Read Isolation and Growth of Primary Cell Cultures from Mouse Protocol]

Category: E Coli Protocols: E coli Nucleic Acid Protocols

Protocol for the isolation of total RNA from E. coli for Microarrays. Includes: Harvesting total RNA; DNase I treatment of RNA samples. - [Read Isolation of Total RNA form E. coli for Microarrays Protocol]

Category: Microarray Protocols: Microarray Analysis Protocols

Protocol for isolation of total RNA from E. coli for microarrays. Includes: Harvesting total RNA; Dnase I treatment of RNA samples. - [Read Isolation of Total RNA From E. Coli for Microarrays Protocol]

Category: Molecular Biology Protocols

Protocol for mRNA amplification. - [Read mRNA Amplification Protocol]