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Actin Staining in Fixed Yeast Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4168

Category: Cytoskeleton Protocols: Actin Myosin Protocols

Phalloidin binds specifically to F-actin, and fluorescent-tagged phalloidin stains the actin skeleton in cells in a manner that is very close to the staining pattern seen using anti-actin antibody. - [Read Actin Staining in Fixed Yeast Cells Protocol]

Cytochrome Oxidase Technique Protocol - http://www.nottingham.ac.uk/pathology/protocols/cox.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for cytochrome oxidase technique, enzyme histochemical stains. - [Read Cytochrome Oxidase Technique Protocol]

Detection of Mycoplasma in Mammalian Cell Cultures Using Fluorescence Microscopy Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4351

Category: Cell Culture Protocols: Cell Culture Contamination Prevention and Removal Protocols

Protocol describes a method for mycoplasma detection using Hoechst 33258, a fluorescent dye that stains DNA. Staining of particulate DNA matter around the cell nucleus (on the cell surface or in the cytoplasm) is indicative of mycoplasma contamination. - [Read Detection of Mycoplasma in Mammalian Cell Cultures Using Fluorescence Microscopy Protocol]

DNA IQ Isolation of Genomic DNA from Stains and Buccal Swabs Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4098

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

In this protocol, the DNA-binding capacity of Wizard MagneSil particles is used to capture and release a consistent amount of DNA (100 ng) across a wide range of samples. At the end of the procedure, the DNA is eluted into 100 µl Elution Buffer to give a final concentration of 1 ng/µl, relieving the need for postpurification DNA quantitation. - [Read DNA IQ Isolation of Genomic DNA from Stains and Buccal Swabs Protocol]

Enzyme Histochemical Stains Protocol - http://www.nottingham.ac.uk/pathology/protocols/nse.html

Category: Neuroscience Protocols: Neuronal Stains Protocols

Protocol for enzyme histochemical stain. - [Read Enzyme Histochemical Stains Protocol]

Measurement of DNA Content Using Propidium Iodide (PI) Staining of Fixed Whole Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4436

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

This protocol describes a method for quantitative measurement of DNA using propidium iodide (PI) staining and flow cytometry. PI stains all double-stranded regions of both DNA and RNA by intercalating between the stacked bases of the double helix. PI cannot penetrate an intact cell membrane; therefore, cells are fixed prior to staining. The ethanol-fixed cells can be stored unstained at 4°C for days, or even weeks, and then stained and analyzed. - [Read Measurement of DNA Content Using Propidium Iodide (PI) Staining of Fixed Whole Cells Protocol]

Negative Stain Electron Microscopy of Microtubules Protocol - http://mitchison.med.harvard.edu/protocols/EM.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of heavy atom stains, structural artifacts such as flattening of the cylindrical microtubule and opening up of microtubules into flat sheets are common. Negative staining is very useful because of its ease, rapidity and lack of requirement for specialized equipment other than that found in a regular EM facility. - [Read Negative Stain Electron Microscopy of Microtubules Protocol]

Simple Staining Protocol - http://www.bact.wisc.edu/Microtextbook/index.php?module=Book&func=displayarticle&art_id=88

Category: Staining Protocols: Simple Staining Protocols

Protocol for simple staining. Smear is stained with a solution of a single dye which stains all cells the same color. Differentiation of cell types or structures is not the objective of the simple stain. However, certain structures which are not stained by this method may be easily seen, for example, endospores and lipid inclusions. - [Read Simple Staining Protocol]

Special Stains in Histology - http://medlib.med.utah.edu/WebPath/HISTHTML/STAINS/STAINS.html

Category: Histology Protocols

Special Stains in Histology. Mucin stains, Melanin stains, etc. - [Read Special Stains in Histology]

Yeast Vital Stains: Calcofluor Staining of Chitin and Bud Scars Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4166

Category: Yeast Protocols: Yeast Staining Protocols

Various vital stains are available for yeast. This protocol describes the staining of chitin and bud. - [Read Yeast Vital Stains: Calcofluor Staining of Chitin and Bud Scars Protocol]

Yeast Vital Stains: DAPI Stain of Nuclear and Mitochondrial DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4163

Category: Yeast Protocols: Yeast Staining Protocols

Protocol describes the staining of nuclear and mitochondrial DNA with DAPI (4',6-diamidino-2-phenylindole). - [Read Yeast Vital Stains: DAPI Stain of Nuclear and Mitochondrial DNA Protocol]

Yeast Vital Stains: Visualizing Mitochondria with DiOC6 or DiIC5 Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4164

Category: Cell Organelle Protocols: Mitochondria Protocols

Protocol describes the visualization of mitochondria with DiOC6 or DiIC5(3). - [Read Yeast Vital Stains: Visualizing Mitochondria with DiOC6 or DiIC5 Protocol]