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12-well Chemotaxis Chamber Protocol - http://www.neuroprobe.com/protocols/AA12.html

12-well Chemotaxis Chamber Protocol. Preparing the Chamber, Preparing and Adding Responding Cells, Removing, Wiping and Staining the Filter. Neuroprobe - [Read 12-well Chemotaxis Chamber Protocol]

5-Bromo-2-Deoxyuridine (BrdU) and 7-Amino-Actinomycin (7-AAD) Staining for Cell Proliferation Assay - http://www.natureprotocols.com/2007/01/22/5bromo2deoxyuridine_brdu_and_7.php

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

The incorporation of 5-bromo-2-deoxyuridine (BrdU) in replicating DNA of proliferating cells can be used to measure their proliferation. This protocol allows the identification of cells which have incorporated BrdU by flow cytometry. - [Read 5-Bromo-2-Deoxyuridine (BrdU) and 7-Amino-Actinomycin (7-AAD) Staining for Cell Proliferation Assay]

ABC Monoclonal Antibody Staining of Dissected Larval and Adult CNS Protocol - http://jfly.iam.u-tokyo.ac.jp/html/manuals/pdf/E_Staining_ItoKei.pdf

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for ABC monoclonal antibody staining of dissected larval and adult CNS. - [Read ABC Monoclonal Antibody Staining of Dissected Larval and Adult CNS Protocol]

Actin Staining in Fixed Yeast Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4168

Category: Cytoskeleton Protocols: Actin Myosin Protocols

Phalloidin binds specifically to F-actin, and fluorescent-tagged phalloidin stains the actin skeleton in cells in a manner that is very close to the staining pattern seen using anti-actin antibody. - [Read Actin Staining in Fixed Yeast Cells Protocol]

AFLP protocol - http://www.msu.edu/user/hazensam/aflp/AFLPprotocolMSU.html

Category: PCR Protocols: AFLP PCR

Restriction Digestion of Genomic DNA , Adapter preparation, Ligation of Adapters. Pre amplification Reactions. Gel electrophoresis, Silver Staining. AFLP Protocol and procedure. - [Read AFLP protocol]

Agarose Gel Electrophoresis PDF - http://www.uni-tuebingen.de/evoeco/files/methods/Electrophoresis.pdf

Category: Molecular Biology Protocols: Agarose Gel Preparation

Agarose Gel Electrophoresis. Pouring of Agarose Gel, Preparation of samples, Gel electrophoresis, Ethidium bromide staining. - [Read Agarose Gel Electrophoresis PDF]

Alcian Blue Staining Technique Protocol - http://www.nottingham.ac.uk/pathology/protocols/alcblue.html

Category: Neuroscience Protocols: Histology Stains Protocols: Mucin Stains Protocols

Protocol for alcian blue staining technique. - [Read Alcian Blue Staining Technique Protocol]

Analysis of Cellular DNA Content by Flow Cytometry Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C8A9C23374B5FAB0610666CCDFB&objectid=6674F58BDEF6DCA5276258FEA4CD534D

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

Simple and universally applicable methods for staining fixed cells are presented, as are methods that utilize detergents and proteolytic treatment to permeabilize cells. Additionally, supravital cell staining with Hoechst 33342, which is primarily used for sorting live cells for subsequent culturing based on DNA-content differences, is also described. Also presented are methods for staining of cell nuclei isolated from paraffin-embedded tissues, and deconvolution of DNA-content-frequency... - [Read Analysis of Cellular DNA Content by Flow Cytometry Protocol]

Analysis of DNA Fragmentation Using Propidium Iodide (PI) Staining After Ethanol Fixation Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4431

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

This protocol provides a method for analysis of DNA fragmentation using flow cytometry after propidium iodide (PI) labeling of fixed apoptotic cells. Flow cytometry reveals apoptotic nuclei in the subdiploid region of the cell cycle histogram... - [Read Analysis of DNA Fragmentation Using Propidium Iodide (PI) Staining After Ethanol Fixation Protocol]

Analysis of Mitochondrial Membrane Potential with the Sensitive Fluorescent Probe JC-1 - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/cossar1/cossariz.htm

Category: Cell Organelle Protocols: Mitochondria Protocols

The technique of JC-1 staining has been developed with the intent to detect DY in intact, viable cells. For this purpose JC-1 acts as a marker of mitochondrial activity, since the formation of J-aggregates, which give red emission, is reversible. Cells with high DY are those forming J-aggregates, thus showing high red fluorescence. On the other hand, cells with low DY are those in which JC-1 maintains (or re-acquire) monomeric form, thus showing only green fluorescence. - [Read Analysis of Mitochondrial Membrane Potential with the Sensitive Fluorescent Probe JC-1]

Analysis of Protein Gels (SDS-PAGE) - http://www.ruf.rice.edu/~bioslabs/studies/sds-page/gellab3.html

Category: Protein Protocols: Protein Electrophoresis

An excellent guide on the analysis of proteins on SDS-PAGE gels, through staining with coomassie blue dye and western blot analysis. Analysis of Protein Gels (SDS-PAGE). David R. Caprette, Rice University. - [Read Analysis of Protein Gels (SDS-PAGE)]

Annexin V Staining Flow Cytometry Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/amfc/data/page1.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Annexin V Staining Flow Cytometry Protocol - a general protocol for staining cell for cytometry analysis. BD PharMingen. - [Read Annexin V Staining Flow Cytometry Protocol]

Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4523

Category: C. elegans Protocols: C. elegans Staining Protocols

Extreme care should be used to identify and verify positive reactions, however, because cross-reactions are common. Counterstaining is essential for examining worms by immunofluorescence and is used to identify the exact cell in which an antigen appears. Methods for counterstaining include labeling all cells with a fluorescent dye that is specific for nucleic acids (e.g., DAPI or propidium iodide) and using GFP driven by tissue-specific promoters. - [Read Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol]

Antibody Addition to Drosophila Specimens and Detection Using Enzyme-Linked Reagents Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4526

Category: Drosophila Protocols

Enzyme-linked reagents give excellent sensitivity and use a simple light microscope for detection. A range of enzymes is available, but for staining in situ, horseradish peroxidase will suit most needs. Diaminobenzidine (DAB) is one of the most sensitive substrates for horseradish peroxidase. It yields an intense brown product that is insoluble in both water and alcohol. It can be made more sensitive by adding metal salts such as cobalt or nickel to the substrate solution. - [Read Antibody Addition to Drosophila Specimens and Detection Using Enzyme-Linked Reagents Protocol]

Antibody Staining of Dissected Gonads Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_antibody.html

Category: C. elegans Protocols: C. elegans Staining Protocols

Protocol for antibody staining of dissected gonads. - [Read Antibody Staining of Dissected Gonads Protocol]

Antigen Retrieval for immunohistochemistry - http://herpesvirus.tripod.com/research/protoc.htm#Antigen%20Retrieval%20for

Category: Immunology: Antigen Retrieval

Antigen Retrieval for immunohistochemistry. Microwave method, Autoclave method. slides and staining. Janos Luka. Eastern Virginia. - [Read Antigen Retrieval for immunohistochemistry]

Antigen Retrieval PDF - http://www.dakousa.com/ishbantigenretr.pdf

Category: Immunology: Antigen Retrieval

Antigen Retrieval. Principles and Technique. A handbook of Immunochemical Staining Methods and techniques. Marc Key - [Read Antigen Retrieval PDF]

Apoptosis Detection Protocol By Single Laser Flow Cytometry - http://cyto.mednet.ucla.edu/Protocols/detection.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

A sensitive method for the detection of apoptosis by single laser flow cytometry. Methodology includes: Staining for detection of apoptosis, Direct Staining Procedure, Indirect Staining Procedure, Protocol for the use of actinomycin D (AD) on samples that were stained with 7-AAD for apoptosis and fixed in formaldehyde. - [Read Apoptosis Detection Protocol By Single Laser Flow Cytometry]

Blocking of Unwanted Non-Specific Staining Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/NonSpecificBlocking.html

Category: Immunohistochemistry Protocols: Blocking Protocols

Protocol for blocking of unwanted non-specific staining. Includes: Blocking of endogenous enzymes; Blocking of endogenous fluorochromes; Blocking endogenous biotin; Blocking of endogenous Fc blocking; Blocking of crossreactive antigens in the tissue. - [Read Blocking of Unwanted Non-Specific Staining Protocol]

Blocking of unwanted non-specific staining in Immunofluorescence. - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/NonSpecificBlocking.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Blocking of unwanted non-specific staining in Immunofluorescence. Blocking of endogenous enzymes, Blocking of endogenous fluorochromes, Blocking endogenous biotin , Blocking of endogenous Fc blocking, Blocking of crossreactive antigens in the tissue. Cattoretti. Columbia University - [Read Blocking of unwanted non-specific staining in Immunofluorescence.]

Blocking Unwanted Non-Specific Staining Protocol - http://www.ihcworld.com/_protocols/general_IHC/ihc_blocking.htm

Category: Immunohistochemistry Protocols: Blocking Protocols

Protocol for blocking of unwanted non-specific staining. Includes: Blocking of endogenous enzymes; Blocking of endogenous fluorochromes; Blocking endogenous biotin; Blocking of endogenous Fc blocking; Blocking of cross reactive antigens in the tissue. - [Read Blocking Unwanted Non-Specific Staining Protocol]

Calcium Flux Analysis Protocol for Flow Cytometry - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/calcium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for calcium flux analysis. Includes: Reagent Preparation; Sample Preparation; Cell Surface Staining; Instrument Set up; Optimizing the Instrument Settings; Recording Data to Apply Compensation; Recording Experimental Data. - [Read Calcium Flux Analysis Protocol for Flow Cytometry]

Calcium Flux: Indo-1 Loading and Sample Staining Procedure for Simultaneous Measurement of Intra Ca2 - http://www.natureprotocols.com/2007/01/29/calcium_flux_indo1_loading_and.php

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Protocol for Calcium Flux: Indo-1 loading and sample staining procedure for simultaneous measurement of intracellular Ca2+. Includes: Preparation of INDO-1; Preparation of Ionomycin. - [Read Calcium Flux: Indo-1 Loading and Sample Staining Procedure for Simultaneous Measurement of Intra Ca2]

Cartilage Staining of Xenopus tropicalis Protocol - http://tropicalis.berkeley.edu/home/gene_expression/cartilage-stain/alcian.html

Category: Xenopus Protocols

Protocol describes the use of a basic water-based dye to stain for acid mucosubstances and acetic mucins located on the cartilage of fixed embryos, permitting the examination of cartilage formation. - [Read Cartilage Staining of Xenopus tropicalis Protocol]

CD90 (Thy-1) Antibody Staining Protocol for Immunohistochemistry - http://www.ihcworld.com/_protocols/antibody_protocols/cd90_pharmingen.htm

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for CD90 (Thy-1) antibody staining for Immunohistochemistry. Includes: Primary Antibody; Antigen Retrieval; Detection Methods; Chromogen Substrate; Counterstain. - [Read CD90 (Thy-1) Antibody Staining Protocol for Immunohistochemistry]