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Acetyl Cholinesterase Enzyme Histochemical Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/cholase.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol stain for acetyl cholinesterase enzyme. - [Read Acetyl Cholinesterase Enzyme Histochemical Stain Protocol]

Acid Phosphatase Histochemical Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/acphos.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for acid phosphatase histochemical stain. - [Read Acid Phosphatase Histochemical Stain Protocol]

Acid Picro Mallory Connective Tissue Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/acidpicro.html

Category: Neuroscience Protocols: Histology Stains Protocols: Connective Tissue Stains Protocols

Protocol for connective tissue stain. Acid Picro Mallory. - [Read Acid Picro Mallory Connective Tissue Stain Protocol]

Adenosine Triphosphatase ATPASE Histochemical Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/atpase.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for adenosine triphosphatase ATPASE histochemical stain. - [Read Adenosine Triphosphatase ATPASE Histochemical Stain Protocol]

Alkaline Phosphatase Histochemical Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/alkphos.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for alkaline phosphatase histochemical stain. - [Read Alkaline Phosphatase Histochemical Stain Protocol]

Analysis of DNA Fragmentation Using Propidium Iodide (PI) Fluorescence of Individual Nuclei Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4430

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

This protocol provides a method for analysis of DNA fragmentation using flow cytometry after propidium iodide (PI) labeling of apoptotic nuclei. Apoptotic nuclei stain less than their normal counterparts because of diffusion of low-molecular-weight fragments out of the cells and/or chromatin condensation. - [Read Analysis of DNA Fragmentation Using Propidium Iodide (PI) Fluorescence of Individual Nuclei Protocol]

Bielschowsky Technique for Senile Plaques Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/BIELSCH.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

A silver stain to demonstrate neurofibrillary tangles, nerve fibers and senile plaques in Alzheimer's disease. The nerve fibers are sensitized with a silver solution. The sections are treated with ammoniacal silver, and then reduced to a visible metallic silver. - [Read Bielschowsky Technique for Senile Plaques Protocol]

Cartilage Staining of Xenopus tropicalis Protocol - http://tropicalis.berkeley.edu/home/gene_expression/cartilage-stain/alcian.html

Category: Xenopus Protocols

Protocol describes the use of a basic water-based dye to stain for acid mucosubstances and acetic mucins located on the cartilage of fixed embryos, permitting the examination of cartilage formation. - [Read Cartilage Staining of Xenopus tropicalis Protocol]

Characterization of Cells by Flow Cytometry Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000018.pdf?pid=PP00000018

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol uses specific antibodies coupled to one of four fluorochromes: fluorescein isothiocyanate (FITC), R-phycoerythrin (PE), peridinin chlorophyll-a (PcP), and allophycocyanin (APC). These fluorochromes can be used simultaneously to stain and analyze the expression patterns of four different proteins in the same sample. The fluorochrome stained cell populations are analyzed using a FACSCalibur dual-laser flow cytometer. - [Read Characterization of Cells by Flow Cytometry Protocol]

Characterization of Cells by Flow Cytometry Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000018.pdf?pid=PP00000018

Category: Immunology: B Cell Protocols

Fluorochromes can be used simultaneously to stain and analyze the expression patterns of four different proteins in the same sample. The fluorochrome stained cell populations are analyzed using a FACSCalibur dual-laser flow cytometer. - [Read Characterization of Cells by Flow Cytometry Protocol]

Congo Red Putcher's Modification Amyloid Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/CONGORED.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

Protocol to demonstrate amyloid deposits in tissue sections. When stained with the Congo Red Stain the amyloid, with the aide of polarizing lenses, will birefringe an apple green color. Under the microscope. - [Read Congo Red Putcher's Modification Amyloid Protocol]

Connective Tissue Stain for Muscle Biopsy Protocol - http://www.nottingham.ac.uk/pathology/protocols/gomori.html

Category: Neuroscience Protocols: Histology Stains Protocols: Connective Tissue Stains Protocols

Protocol for connective tissue stain for muscle biopsy. Gomori Trichome. - [Read Connective Tissue Stain for Muscle Biopsy Protocol]

Connective tissue Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/vangies.html

Category: Neuroscience Protocols: Histology Stains Protocols: Connective Tissue Stains Protocols

Protocol for connective tissue stain. - [Read Connective tissue Stain Protocol]

Detection of ß-Galactosidase and Alkaline Phosphastase Activities in Tissue Protocol - http://genetics.med.harvard.edu/~cepko/protocol/xgalplap-stain.htm

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for detection of ß-Galactosidase and alkaline phosphastase activities in tissue. - [Read Detection of ß-Galactosidase and Alkaline Phosphastase Activities in Tissue Protocol]

Dye Filling to Stain Amphid and Phasmid Neurons Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=9

Category: C. elegans Protocols: C. elegans Staining Protocols

Protocol for dye filling to stain amphid and phasmid neurons. - [Read Dye Filling to Stain Amphid and Phasmid Neurons Protocol]

Dye Filling to Stain Amphid and Phasmid Neurons Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=9

Category: Neuroscience Protocols: Neuronal Stains Protocols

Protocol for dye filling to stain amphid and phasmid neurons. - [Read Dye Filling to Stain Amphid and Phasmid Neurons Protocol]

Elastic and Connective Tissue Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/evg.html

Category: Neuroscience Protocols: Histology Stains Protocols: Connective Tissue Stains Protocols

Protocol for elastic and connective tissue stain. Elastic Van Gieson (EVG). - [Read Elastic and Connective Tissue Stain Protocol]

Elastic Picro Sirius Red Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/epsr.html

Category: Neuroscience Protocols: Histology Stains Protocols: Connective Tissue Stains Protocols

Protocol for elastic picro sirius red connective tissue stain. - [Read Elastic Picro Sirius Red Stain Protocol]

Embryo Lysates & Immunoprecipitation Protocol - http://spot.colorado.edu/~klym/Methods/Precipitation.htm

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol for embryo lysates and immunoprecipitation: protein A agarose & protein A magnetic beads. Includes: Embryo lysates; Immunoprecipitation with protein A agarose; Immunoprecipitation with protein A magnetic beads for silver stain/sequencing analysis. - [Read Embryo Lysates & Immunoprecipitation Protocol]

Enzyme Histochemical Stains Protocol - http://www.nottingham.ac.uk/pathology/protocols/nse.html

Category: Neuroscience Protocols: Neuronal Stains Protocols

Protocol for enzyme histochemical stain. - [Read Enzyme Histochemical Stains Protocol]

Fixation, permeabilization and immunostaining of cells - http://www.lamondlab.com/pdf/Fix&Stain.pdf

Category: Immunology: Immunocytochemistry Protocols

Fixation, permeabilization and immunostaining of cells. Lamond Lab, Univ. Dundee - [Read Fixation, permeabilization and immunostaining of cells]

Fluorescent Staining of Cells - http://ylwang.umassmed.edu/protocol/cfs/stain.htm

Category: Molecular Imaging: Immunofluorescence Microscopy

Fluorescent Staining of Cells protocol. Yu-li Wang Lab. Fluorescent Phalloidin Staining, Immunofluorescence Staining and procedures for staining. - [Read Fluorescent Staining of Cells]

FM 4-64 Labeling of Yeast Vacuole Membranes Protocol - http://www.colorado.edu/MCDB/odorizzilab/FM464labeling.pdf

Category: Microscopy Protocols

FM 4-64 is a lipophilic styryl dye and a vital stain: it fluoresces only in living cells, so cells cannot be fixed then stained nor stained then fixed. You must stain and observe living cells. FM 4-64 does not permeate cell membranes but, instead, intercalates into the plasma membrane is then taken into the cells by endocytosis. - [Read FM 4-64 Labeling of Yeast Vacuole Membranes Protocol]

General Stain for Toludine Blue Protocol - http://www.nottingham.ac.uk/pathology/protocols/tolblue.html

Category: Neuroscience Protocols: Histology Stains Protocols: Mucin Stains Protocols

Protocol for toludine blue general stain. - [Read General Stain for Toludine Blue Protocol]

Gills Haematoxylin Stain Solution Protocol - http://www.nottingham.ac.uk/pathology/protocols/gills.html

Category: Histology Stains Protocols

Protocol for Gills Haematoxylin stain solution. - [Read Gills Haematoxylin Stain Solution Protocol]