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Cytokine ELISPOT (Enzyme-linked ImmunoSPOT) Protocols - http://www.ebioscience.com/ebioscience/appls/ELISPOT.htm

Category: Signalling Signal Transduction Protocols: Cytokine Protocols

Cytokine Enzyme-linked immunosorbent spot (ELISPOT) assays. Cytokine ELISPOT Buffers and procedure protocol. - [Read Cytokine ELISPOT (Enzyme-linked ImmunoSPOT) Protocols]

Embryo Lysates & Immunoprecipitation Protocol - http://spot.colorado.edu/~klym/Methods/Precipitation.htm

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol for embryo lysates and immunoprecipitation: protein A agarose & protein A magnetic beads. Includes: Embryo lysates; Immunoprecipitation with protein A agarose; Immunoprecipitation with protein A magnetic beads for silver stain/sequencing analysis. - [Read Embryo Lysates & Immunoprecipitation Protocol]

Estimating the Yield of DIG-labeled Nucleic Acids Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_65-70.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

Protocol for estimating the yield of DIG-labeled nucleic acids. Includes: Estimating the yield in a spot test with a DIG-labeled control. - [Read Estimating the Yield of DIG-labeled Nucleic Acids Protocol]

GFP-Labelled Crest Transplant Protocol - http://spot.colorado.edu/~klym/Methods/cresttransplant.htm

Category: Xenopus Protocols

Protocol for GFP-labelled crest transplant. - [Read GFP-Labelled Crest Transplant Protocol]

How to Use Spot Tests to Determine Mutagen Sensitivity - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20spot%20tests%20to%20determine%20mutagen%20sensitivity.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use spot tests to determine mutagen sensitivity. - [Read How to Use Spot Tests to Determine Mutagen Sensitivity]

Making and Injecting Xenopus Embryos Protocol - http://spot.colorado.edu/~klym/Methods/embryos.htm

Category: Xenopus Protocols: Xenopus Injection Protocols

Protocol for making and injecting Xenopus embryos. Includes: Injections; Egg Activation and Inversion Experiments; - [Read Making and Injecting Xenopus Embryos Protocol]

Protocols for microarray development in use in the Vodkin Laboratory - http://soybeangenomics.cropsci.uiuc.edu/protocols/index.html

Category: DNA Microarray Protocols

Reverse transcriptase labeling with Cy3/Cy5, Staining of total DNA on slides, construction, Laser scanning, Spot quantitation, Statistics, sample tracking. Vodkin Laboratory. U.Illinois. - [Read Protocols for microarray development in use in the Vodkin Laboratory]

Xenopus Chromatin immmunoprecipitation Protocol - http://spot.colorado.edu/~klym/Methods/XChIP.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus chromatin immunoprecipitation. - [Read Xenopus Chromatin immmunoprecipitation Protocol]

Xenopus DNA Fishing Protocol - http://spot.colorado.edu/~klym/Methods/fishing.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus DNA fishing. Includes: DNA-agarose coupling reaction; PROTEIN BINDING REACTION; OLIGO ANNEALING. - [Read Xenopus DNA Fishing Protocol]

Xenopus RNA Isolation Protocol - http://spot.colorado.edu/~klym/Methods/RNAisolation.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus RNA isolation. - [Read Xenopus RNA Isolation Protocol]

Articles (1-3 of 3)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.