spore Protocols

Search results for: spore

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Assessment of Magnaporthe grisea Mating Type by Spore PCR - http://www.fgsc.net/fgn42/xu.html

Category: Fungi Protocols: Fungi Genetics Protocols

PCR protocol which uses M. grisea conidia directly for PCR analysis without extraction of DNA. - [Read Assessment of Magnaporthe grisea Mating Type by Spore PCR]

How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab - http://www.fgsc.net/neurosporaprotocols/How%20to%20avoid%20pitfalls%20and%20keep%20out%20of%20trouble.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to avoid pitfalls and keep out of trouble in the Neurospora lab. Includes: Culture, incubation, storage, and disposal; Stock preservation; Spore and colony counts; Making crosses and recovering progeny; Use of heterokaryons for allelism tests; Use of unordered asci to estimate the relation of translocation breakpoints to centromeres. - [Read How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab]

How to Distinguish a Spore Killer From Alternative Causes of Ascospore Abortion - http://www.fgsc.net/neurosporaprotocols/How%20to%20distinguish%20a%20Spore%20kill.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to distinguish a Spore killer from alternative causes of ascospore abortion. - [Read How to Distinguish a Spore Killer From Alternative Causes of Ascospore Abortion]

How to Use Spore Killer for Distinguishing 1st- and 2nd-Division Segregation with Unordered Asci - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20Spore%20killer%20for%20distinguishing%201st-%20and%202nd-division%20segregation%20with%20unordered%20asci.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use Spore killer for distinguishing 1st- and 2nd-division segregation with unordered asci. - [Read How to Use Spore Killer for Distinguishing 1st- and 2nd-Division Segregation with Unordered Asci]

Random Spore Analysis in Yeast Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4162

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Random spore analysis is used to examine meiotic recombination without performing tetrad analysis. - [Read Random Spore Analysis in Yeast Protocol]

Spore Germination Protocol - http://www-rcf.usc.edu/~forsburg/spores.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for spore germination. This procedure is typically used for the isolation and preparation of spores from a diploid strain heterozygous for a marked disruption (e.g., yfg1::his3+) Inoculation of the spore population into minimal medium lacking the nutritional supplement corresponding to the disruption marker (e.g., minimal medium lacking histidine) allows only the disruption spores to germinate. - [Read Spore Germination Protocol]