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C2 Preparation of pre-formed iodixanol gradients for mammalian cells. - http://www.axis-shield.com/densityhome/optiprep/C02.pdf

Category: Cell Biology and Cell Culture

Techniques on how to create gradients of iodixanol for the fractionation of mammalian cells. These gradients can be generated as pre-formed discontinuous or continuous gradients. These gradients are invariably run in swinging-bucket rotors in low-speed centrifuges. - [Read C2 Preparation of pre-formed iodixanol gradients for mammalian cells.]

Cell Fractionation protocol Usingt Low Speed Separaton of Cells - http://homepages.gac.edu/~cellab/chpts/chpt3/ex3-4.html

Category: Cell Biology and Cell Culture

Cell fractionation protocol using low speed seperation. - [Read Cell Fractionation protocol Usingt Low Speed Separaton of Cells]

Centrifuge Safety - High Speed Hazards - http://www.ehs.wsu.edu/Factsheets/FAQCentrifuge.asp

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Centrifuge Safety - High Speed Hazards. Safe Centrifuge Use , Care and Maintenance, and maintaining a log book for centrifuges. EHS Washington state Univ. - [Read Centrifuge Safety - High Speed Hazards]

DNA Miniprep Isolation from Plants Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1079

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

The original maize DNA miniprep protocol is used extensively for many plant species and different tissues. This slightly modified version is acceptable for most DNA extractions. The procedure has the advantage of speed and its use of inexpensive reagents. - [Read DNA Miniprep Isolation from Plants Protocol]

Fragmentation of DNA by Nebulization Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4539

Category: Cloning Protocols

Protocol describes a method for DNA fragmentation by nebulization, in which the fine mist created by forcing a DNA solution through a small hole in the nebulizer unit is collected. The size of the fragments obtained by nebulization is determined chiefly by the speed at which the DNA solution passes through the hole, altering the pressure of the gas blowing through the nebulizer, the viscosity of the solution, and the temperature. - [Read Fragmentation of DNA by Nebulization Protocol]

Manual Microdissection - http://cgap-mf.nih.gov/Protocols/Microdissection/ManualMicrodissection.html

Category: Primary Cell Isolation and Culture Protocols

There are several manual methods that can be used to perform tissue microdissection. Techniques using hand-held tools as well as mechanical micromanipulator-based approaches have been described. However, speed and precision are the most important parameters and any method that achieves these is adequate. Investigators should also expect to invest time initially by practicing on 10 to 20 cases to begin to feel comfortable with the technique. - [Read Manual Microdissection]

Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport - http://www.bio.com/protocolstools/protocol.jhtml?id=p1841

Category: Yeast Protocols

Protocol for microsome high speed pellet and cytosol preparation for endoplasmic reticulum to golgi transport assay in Yeast. - [Read Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport]

Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport Ass - http://www.bio.com/protocolstools/protocol.jhtml?id=p1841

Category: Cell Organelle Protocols: Microsome Protocols

Protcol relating to microsome high speed pellet and cytosol preparation for endoplasmic reticulum to golgi transport assay in yeast. Includes spheroplast formation. - [Read Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport Ass]

Preparation and Loading of Protein Samples for Microinjection Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4656

Category: Microinjection Protocols: Microinjection of Proteins Protocols

his protocol provides methods for the preparation of protein samples and for loading them into pulled microinjection pipettes. Stock solutions of proteins are thawed, diluted (if desired), centrifuged at high speed to remove aggregates, and kept on ice until loading. Loading into micropipettes can be done using either a "front-loading" or a "backfilling" procedure. - [Read Preparation and Loading of Protein Samples for Microinjection Protocol]

Removal of Small Fragments of Nucleic Acid from Preparations of Plasmid DNA by Precipitation with Li - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3918

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

High-molecular-weight RNA and proteins can be precipitated from preparations of plasmid DNA by high concentrations of LiCl and removed by low-speed centrifugation. - [Read Removal of Small Fragments of Nucleic Acid from Preparations of Plasmid DNA by Precipitation with Li]

Articles (1-3 of 3)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

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