spectrometer Protocols

Category: Mass Spectrometry Protocols

Fast Evaporation Method for MALDI sample preparation protocol. Inserting into MALDI mass spectrometer and acquiring a spectrum. EMBL Bioanalytical Research Group. Wilm. - [Read MALDI Sample Preparation - Fast Evaporation Method]

Category: Protein Protocols: Protein Quantitation Concentration Protocols

UV Absorbance 280 nm Protein Determination. Simple and quick method to accurately quantitate total protein in purified material or approximately quantitate total protein in crude lysates or partial purified material. Quantitation of the amount of protein in a solution is possible in a simple spectrometer.  Absorption of radiation in the near UV by proteins depends on the Tyr and Trp content (and to a very small extent on the amount of Phe and disulfide bonds).  Dr. Mario Lebendiker - [Read UV Absorbance 280 nm Protein Determination]