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Practical PCR Genotyping Protocols for Plasmodium vivax using Pvcs and Pvmsp1 - http://bmc.ub.uni-potsdam.de/1475-2875-4-20/

Category: Genetics and Genomics: Genotyping Protocols: PCR Genotyping

Plasmodium vivax is the second most prevalent malaria parasite affecting more than 75 million people each year, mostly in South America and Asia. In addition to major morbidity this parasite is associated with relapses and a reduction in birthweight. Practical PCR genotyping protocols based on polymorphic loci present in two P. vivax genetic markers. - [Read Practical PCR Genotyping Protocols for Plasmodium vivax using Pvcs and Pvmsp1]

Preparation of Bacterial Glycerol Stocks, Thawing of Stocks - http://sbio.uct.ac.za/Sbio/documentation/Glycerol%20stocks.html

Category: Buffers Media, and Solutions: Bacterial Solutions

Preparation of Bacterial Glycerol Stocks, Thawing of Stocks. South African Structural Biology Initiative. - [Read Preparation of Bacterial Glycerol Stocks, Thawing of Stocks]

Preparation of Bacterial Growth Broths and Agar, Antibiotics and IPTG - http://sbio.uct.ac.za/Sbio/documentation/Bacterial%20Media%20and%20solutions.html

Category: Buffers Media, and Solutions: Bacterial Solutions

Preparation of Bacterial Growth Broths and Agar, Antibiotics and IPTG. South African Stuctural Biology Initiative. - [Read Preparation of Bacterial Growth Broths and Agar, Antibiotics and IPTG]

Selection of Transfected Suspension Cells - http://www.musc.edu/BCMB/ceramide/protocols/0008.html

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

hygromycin, neomycin selection of stable cells in suspension. Suprya Jayadev / Dr.Hannun Dr.Obeid Lab, BCMB, South Carolina. - [Read Selection of Transfected Suspension Cells]

Articles (1-2 of 2)

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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