User Panel

My Panel

Bookmark Science Articles

solutions Protocols

Search results for: solutions

Protocols » Search Results

Featured Search Results Links

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Search Results Protocol Links Sort by: Hits | Alphabetical

2 Hybrid System TRAFO Protocol - http://www.umanitoba.ca/faculties/medicine/biochem/gietz/2HS.html

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Yeast Two-Hybrid System. TRAFO Solutions. Gietz Lab - [Read 2 Hybrid System TRAFO Protocol]

Agarose Gel Preparation Protocol - http://fp.bio.utk.edu/mycology/Techniques/mt-agarose_gels.htm

Category: Molecular Biology Protocols: Agarose Gel Preparation

Solutions for Agarose gel prepartion, Selection of Appropriate Gel Size and Composition. Mycology Lab University of Tennessee - [Read Agarose Gel Preparation Protocol]

Antibiotic solutions - http://molbiol.ru/eng/protocol/01_05.html

Category: Microbiology: Antibiotics Concentration in Media LB

Working concentrations and stock solutions for antibiotics, preparation of stock solutions for Ampicillin, Kanamycin and others. Practical Molecular Biology - [Read Antibiotic solutions]

Antibiotics - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Materials/antibiotics.html

Category: Microbiology: Antibiotics Concentration in Media LB

Antibiotic stock solutions preparation, Storage conditions for antibiotics, Working Concentrations for high copy plasmids. Kay Schneitz Univ Zurich. - [Read Antibiotics]

Antibiotics. and Antibiotics Stock Calculator Tools - http://molbiol.edu.ru/eng/protocol/01_05.html

Category: Microbiology: Antibiotics Concentration in Media LB

Working concentrations and stock solutions and which antibiotics are light sensitive. Preparation of stock solutions and calculator tool to calculate concentrations for stock solutions. Practical Molecular Biology. - [Read Antibiotics. and Antibiotics Stock Calculator Tools]

Arabidopsis Tissue Culture Stock Solutions and Media - http://wheat.pw.usda.gov/~lazo/methods/lazo/tissue.html

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

Arabidopsis Tissue Culture Stock Solutions and Media. Lazo Lab. MS MEDIUM FOR ARABIDOPSIS. B5 MEDIUM FOR ARABIDOPSIS. 10X Micronutrients. - [Read Arabidopsis Tissue Culture Stock Solutions and Media]

Bacterial Media Solutions and Stocks - http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid11.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

agar, Ampicillin Stock, Alkaline Lysis Solution, SOB, SOC media, and X-gal stock solutions. Donis Keller. - [Read Bacterial Media Solutions and Stocks]

Bacterial Solutions - http://www.uhmc.sunysb.edu/bioscience/methods/bacteria_methods/bacteria_methods.htm

Category: Buffers Media, and Solutions: Bacterial Solutions

Bacterial Solutions. Stony Brook Univ. Hospital. - [Read Bacterial Solutions]

Blocking Solutions Protocols - http://icg.cpmc.columbia.edu/cattoretti/Protocol/main_page_reagents.html#Blocking%20solutions

Category: Immunohistochemistry Protocols: Blocking Protocols

Protocols for blocking solutions. Includes: Endogenous peroxidase blocking solution (H2O2-Azide); Non-specific antibody background blocking solution: swine serum; Non-specific antibody background blocking solution: MILK; Enzymatic antigen retrieval solution; Unconjugated, biotin- and fluorochrome-conjugated antibody (primary) solutions; Unconjugated, biotin- and fluorochrome-conjugated antibody (secondary) solutions; Enzyme-conjugated secondary antibody solutions.; etc.. - [Read Blocking Solutions Protocols]

Bradford Assay Method - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Biochemistry/Bradford.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue to protein. There is no interference from cations nor from carbohydrates such as sucrose.
Detergents such as sodium dodecyl sulfate and triton x-100 can interfere with the assay, as well as strongly alkaline solutions.

Includes a general overview of the procedure and preparation of the standards in the protocol. - [Read Bradford Assay Method]

Buffer calculator tool - http://molbiol.ru/eng/protocol/01_07.html

Category: Buffers Media, and Solutions

Buffer calculator tool for commonly used buffer compound solutions. Making different volumes? use this! (Practical Molecular Biology) - [Read Buffer calculator tool]

Buffer Solutions and How they work... - http://www.chemguide.co.uk/physical/acidbaseeqia/buffers.html

Category: Buffers Media, and Solutions: Buffer Theory

This page describes simple acidic and alkaline buffer solutions and explains how they work. - [Read Buffer Solutions and How they work...]

Common Buffers - http://wheat.pw.usda.gov/~lazo/methods/lazo/buffer.html

Category: Buffers Media, and Solutions

Recipes for commonly used buffer solutions. (Gerard R. Lazo) - [Read Common Buffers]

Common DNA solutions. - http://www.rickhershberger.com/bioactivesite/lab/recipes.htm

Category: Buffers Media, and Solutions: Buffer Recipes for DNA Work

Common DNA solutions.Dr. Rick Hershberger - [Read Common DNA solutions.]

Concentrating and Desalting Nucleic Acids with Microconcentrators Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4457

Category: Nucleic Acid Purification Protocols

Ultrafiltration is an alternative to ethanol precipitation for the concentration and desalting of nucleic acid solutions. It requires no phase change and is particularly useful for dealing with very low concentrations of nucleic acids. This protocol describes the use of the Microcon cartridge, a centrifugal ultrafiltration device, to concentrate and desalt nucleic acid solutions. - [Read Concentrating and Desalting Nucleic Acids with Microconcentrators Protocol]

COS Cell Transfection Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C5.html

Category: Transfection Protocols

COS cell transfection protocol. Includes solutions: DEAE Dextran and 1000X Chloroquine - [Read COS Cell Transfection Protocol]

Electron Microscopy Protocols detailed - http://synapses.mcg.edu/lab/howto/protocols/physiology.htm

Category: Molecular Imaging: Electron Microscopy Protocols

Electron Microscopy Protocols. LTP Physiology Protocol. Solutions. Standard ACSF is (in mM) NaCl, 117; KCl, 5.3; MgSO4, 1.3; NaH2PO4, 1; NaHCO3, 26; ... Electron Microscopy Protocols - [Read Electron Microscopy Protocols detailed]

ELISPOT Protocol Detailed PDF - http://www.biolegend.com/images/Protocols/BioLegend%20ELISPOT%20protocol.pdf

Category: ELISA Protocols: ELISpot Protocols

Coat the Plate, Block the Plate, Add Detection Antibody, Solutions and buffers for ELISpot. Biolegend. - [Read ELISPOT Protocol Detailed PDF]

EMBL Cloning protocols - http://www.embl-hamburg.de/display?file=~geerlof/webPP/protocoldb/Cloning/pdb_cloning.html

Category: DNA Protocols: DNA Cloning

Preparation of oligo solutions PCR experiments, Digestion of insert DNA, Digestion and dephosphorylation of vector DNA, Ligation of DNA fragments with sticky ends, Ligation of DNA fragments with blunt ends, Preparation of chemically competent E. coli - [Read EMBL Cloning protocols]

Fission Yeast Media Protocol - http://www-rcf.usc.edu/~forsburg/media.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Protocol for fission yeast media. Includes: YES (yeast extract with supplements) for rich complete media; YSO, for modified rich media; EMM (Edinburgh minimal medium) for minimal selective media; stock solutions to make up minimal media; MB, a very stringent minimal for transformation; Sporulation media; ME (malt extract); SPAS; EMMGlut. - [Read Fission Yeast Media Protocol]

Fixing Attached Cells in Paraformaldehyde Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4294

Category: Cell Culture Protocols: Cell Fixing Protocols

Treating cells with paraformaldehyde leads to the establishment of chemical cross-links between free amino groups. When the cross-links join different molecules, a latticework of interactions occurs that holds the overall architecture of the cell together. Commercial formaldehyde solutions are not recommended, because they lack the advantages of using a variable-length polymer, and the cells will simultaneously be fixed with the alcohol (usually methanol). - [Read Fixing Attached Cells in Paraformaldehyde Protocol]

Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC Protocol - http://mitchison.med.harvard.edu/protocols/flowcell.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for flow cell assays with microtubules. Includes: Solutions & Supplies; VE-DIC Assays; Motility; Dynamics; Fluorescence Assays; Oxygen Scavenging; Assays. - [Read Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC Protocol]

Genetic Engineering with PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3836

Category: PCR Protocols

Method describes how to modify the termini of PCR products by introducing restriction sites and other features. To reduce the chance of contamination with exogenous DNAs, prepare and use a special set of reagents and solutions for PCR only. Bake all glassware for 6 hours at 150°C and autoclave all plasticware. - [Read Genetic Engineering with PCR Protocol]

Genetic Engineering with PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3836

Category: PCR Protocols: PCR Optimization

Growing Adherent Cells on Coverslips or Multiwell Slides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4289

Category: Cell Culture Protocols

Glass is an excellent substrate for most tissue-culture-adapted cells and is compatible with all fixing and staining solutions. Glass coverslips in tissue-culture dishes or in 24-well multiwell plates are suitable carriers, as are multiwell slides. For high-resolution studies, choose glass coverslips of the highest available grade; #1 or #1.5 coverslips are the appropriate thickness. - [Read Growing Adherent Cells on Coverslips or Multiwell Slides Protocol]

Articles (1-8 of 8)

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

Thionin Staining

Thionin Staining method.

Thionin Method

Thionin Method and protocol for staining cells.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.