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A Sealed Preparation for Long-Term Observations of Cultured Cells: Support Slide Construction - http://www.cshprotocols.org/cgi/content/extract/2007/1/pdb.ip26

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Protocol on the details required of support slide construction for a sealed preparation for long-term observations of cultured cells. - [Read A Sealed Preparation for Long-Term Observations of Cultured Cells: Support Slide Construction]

Additional slide pre-treatment for mapping smaller insert clones - http://www.sanger.ac.uk/HGP/methods/cytogenetics/additional_treatment.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Additional slide pre-treatment for mapping smaller insert clones for FISH. Sanger Institute. - [Read Additional slide pre-treatment for mapping smaller insert clones]

Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative - http://info.med.yale.edu/genetics/ward/tavi/CCKmethod.html

Category: Cytogenetics Protocols

Protocol for color changing karytoping (CCK): an M-FISH/SKY alternative. Includes: Slide preparation and hybridization; Probes, dyes and antibodies; Probe detection and image capture; Image processing. - [Read Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative]

COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL - http://users.ugent.be/~fspelema/neubla/protocol/cgh.htm

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

SAVING OF DAPI-IMAGES, SLIDE PRETREATMENT, PROBE PREPARATION, PROBE DETECTION, washing blocking detection, Counterstaining with DAPI. Institute of Pathology,Humboldt-University of Berlin - [Read COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL]

Comparative Genomic Hybridization PDF - http://www.utoronto.ca/cancyto/protocols_software/CGH_protocol.pdf

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

Excellent Detailed CGH Comparative Genomic Hybridization Protocol. Squire Lab, Univ. Toronto. Includes: Labeling, Gel Interpretation, Slide Pre-Treatment and Denaturation, Probe Denaturation, Hybridization and Post Hybridization Washes and Detection, Tips - [Read Comparative Genomic Hybridization PDF]

Culture Chambers for Live-Cell Imaging - http://www.microscopyu.com/articles/livecellimaging/culturechambers.html

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Specimen chambers have had many designs published over the years describing systems that offer excellent optical properties while allowing specimens to be maintained for varying amounts of time. Ranging in complexity from the simple preparation of a sealed coverslip on a microscope slide to sophisticated perfusion chambers that enable tight control of virtually all environmental variables culture chambers are designed to to allow living specimens to be observed with minimal invasion at high res. - [Read Culture Chambers for Live-Cell Imaging]

Cytogenetic Protocol Blood Preps - http://www.jax.org/cyto/blood_preps.html

Category: Cytogenetics Protocols

Cytogenetic protocol for blood preps. Includes: Preparation of materials; Culture Procedure - Use aseptic technique throughout procedure until harvest; Harvest; Slide making; Collecting blood by tail vein method; PHA Dose Response. - [Read Cytogenetic Protocol Blood Preps]

DNA Precipitation and Hybridization FISH Protocol - http://www.riedlab.nci.nih.gov/publications/DNA%20Precip%20&%20Hybrid%20FISH.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for DNA precipitation and hybridization (FISH). Includes: Precipitation; Slide Denaturation and Hybridization. - [Read DNA Precipitation and Hybridization FISH Protocol]

FISH Guide and Troubleshooting - http://info.med.yale.edu/genetics/ward/tavi/FISHguide.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

FISH Guide and Troubleshooting. Includes: Cytogenetic techniques; Slide preparation; When do chromosomes spread; Cell suspensions; G-banding; Cell fixatives; Slide preparation factors. - [Read FISH Guide and Troubleshooting]

Fixation and Separation of Arabidopsis Leaf Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3DM03FJWU3MGILNR3FQLMSFEWHUWBNQIV0?id=p1095

Category: Plant Biology Protocols

Protocol for the fixation and seperation of Arabidopsis leaf cells. Includes: Sample Preparation; Slide Preparation. - [Read Fixation and Separation of Arabidopsis Leaf Cells Protocol]

G-Banding Observations - http://info.med.yale.edu/genetics/ward/tavi/slide08.html

Category: Cytogenetics Protocols

G-Banding observations. Includes: Slide storage; Fixative composition and trypsin treatment in G-banding; Banding of chemically aged slides. - [Read G-Banding Observations]

Hybridization Protocol for Hybridizing fluorescent cDNA to slide - http://research.nhgri.nih.gov/microarray/hybridization.shtml

Category: DNA Microarray Protocols

Hybridization Protocol for Hybridizing fluorescent cDNA to slide. - [Read Hybridization Protocol for Hybridizing fluorescent cDNA to slide]

Labeled Probe Hybridization Procedure - http://pga.tigr.org/sop/M005_1a.pdf

Category: DNA Microarray Protocols

This protocol describes the hybridization of a Cy labeled cDNA probe (mix of Cy3 and Cy5) onto coated slide spotted with PCR amplified cDNA. TIGR Microarray Protocols. - [Read Labeled Probe Hybridization Procedure]

Laser Capture Microdissection (LCM) - http://cgap-mf.nih.gov/Protocols/Microdissection/LCM.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

LCM utilizes an infrared laser integrated into a standard microscope. A transparent cap is attached to a thermoplastic transparent membrane which lies directly on the surface of a routinely prepared tissue section on a glass slide. The investigator examines the tissue section microscopically and activates the laser when the desired cells underlie the target. This in turn activates the membrane with subsequent binding and procurement of the cells of interest. - [Read Laser Capture Microdissection (LCM)]

Low-melt Polyester Embedding Protocol - http://cgap-mf.nih.gov/Protocols/Tissues/TissueProtocols/LowMeltPolyester.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols: Tissue Preparation for LCM Protocols

Protocol for low-melt polyester embedding. Includes: Large Tissue Specimens; Small Tissue Specimens and Biopsies; Histology Slide Cutting Procedure. - [Read Low-melt Polyester Embedding Protocol]

Microarrays a Complete Manual PDF - http://research.nhgri.nih.gov/microarray/Protocols.pdf

Category: DNA Microarray Protocols

Microarray fabrication, coating, slide blocking, printing microarrays, RNA extraction, Sample Labeling for analysis, hybridization, complete protocol. NHGRI - [Read Microarrays a Complete Manual PDF]

Mitotic Chromosome from Cell Cultures Protocol - http://www.jax.org/cyto/culture_prep.html

Category: Cytogenetics Protocols

Protocol for mitotic chromosome from cell cultures. Includes: Colchicine Treatment; Monolayer cultures; Cell Suspensions; Cell Harvest; Chromosome Slide Preparation. - [Read Mitotic Chromosome from Cell Cultures Protocol]

Monitoring Protein Phosphorylation for the Ligand Screens PPT - http://www.signaling-gateway.org/aboutus/supplement/AM2003/10a_Mumby.ppt

Category: Protein Protocols: Protein Phosphorylation Protocols

A powerpoint slide demonstrating a method for Monitoring Protein Phosphorylation for Ligand Screens. Mumby. signaling-gateway.org - [Read Monitoring Protein Phosphorylation for the Ligand Screens PPT]

Optimized Protocol for Mounting Tissue Sections PDF - http://arctur.com/research_portal/images/Application%20Notes/pen_membrane_protocol_9.pdf

Category: Histology Protocols: Tissue Sample Sectioning

Generally Required Materials and Equipment for Tissue Sectioning. Sectioning and Slide Preparation Procedure onto Metal-Framed PEN Membrane Slides. Arcturus. - [Read Optimized Protocol for Mounting Tissue Sections PDF]

Preparation of Chromosome Spreads - http://www.le.ac.uk/bl/phh4/prochrom.htm

Category: Cytogenetics Protocols

An ideal method of tissue preparation ensures both good specimen morphology and that the target molecules are in the optimum state for probe access and hybridization. DNA:DNA in situ hybridization is usually carried out on chromosome spread preparations where chromosome and nuclei are released from cells and spread on a glass microscope slide. This method yields well separated and enlarged chromosomes with good morphology which can be analyzed in transmitted light or fluorescence microscopes. - [Read Preparation of Chromosome Spreads]

Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol - http://www.riedlab.nci.nih.gov/publications/Slide%20Pretreatment.pdf

Category: Cytogenetics Protocols

Protocol for pretreatment of chromosome slides for FISH/CGH/SKY. - [Read Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol]

Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol - http://www.riedlab.nci.nih.gov/publications/Slide%20Pretreatment.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for the pretreatment of chromosome slides for FISH/CGH/SKY. - [Read Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol]

Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol - http://www.riedlab.nci.nih.gov/publications/Slide%20Pretreatment.pdf

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

Protocol for pretreatment of chromosome slides for FISH/CGH/SKY. - [Read Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol]

Sectioning Mouse Embryos Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4703

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol provides methods and tips for sectioning mouse embryos and transferring the sections to a microscope slide. - [Read Sectioning Mouse Embryos Protocol]

SKY/FISH of Previously G-Banded Slides Protocol - http://www.riedlab.nci.nih.gov/publications/SKY-FISH%20G_Banded_slides.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for SKY/FISH of previously G-banded slides. Includes: G-Band Slide Pretreatment; G-Band Slide Denaturation and Hybridization. - [Read SKY/FISH of Previously G-Banded Slides Protocol]

Articles (1-10 of 10)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.