shotgun Protocols

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Generation of a Library of Randomly Overlapping DNA Inserts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4081

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

Shotgun sequencing of a large segment of DNA involves random fragmentation of the target region into smaller segments that are subsequently cloned into a bacteriophage M13 vector. The goal is to create a library of overlapping clones that provide at least fivefold coverage over the entire length of the target fragment. - [Read Generation of a Library of Randomly Overlapping DNA Inserts Protocol]

Shotgun Library Construction Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/ShotgunLibraryConstruction%28EJSVersion-050511%29.pdf

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

Protocol for shotgun library construction. - [Read Shotgun Library Construction Protocol]