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Absorbance Assay 205 nm - http://www.ruf.rice.edu/~bioslabs/methods/protein/abs205.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Absorbance assay at 280 nm. This method is just as convenient as for absorbance at 280 nm. It may be preferred if there is excessive contamination by nucleic acids, since nucleic acids absorb very little radiation at 205 nm. Setting the wavelength is a bit tricky since 205 nm is right on the shoulder of the protein peak. - [Read Absorbance Assay 205 nm]

AFLP protocol Wolf Lab - http://bioweb.usu.edu/wolf/aflp_protocol.htm

Category: PCR Protocols: AFLP PCR

Restriction and Ligation reactions, Setting up the reactions, AFLP PCR reactions protocols. Analyzing the data using Genescan. Paul G. Wolf, Utah State Univ. - [Read AFLP protocol Wolf Lab]

Microinjecting C.elegans Worms Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=5

Category: C. elegans Protocols: C. elegans Injection Protocols

Protocol for microingecting C.elegans worms. Includes: Making the DNA solution; Setting up the scope, loading the needle, mounting, and breaking the needle; Mounting worms on an injection pad; Injecting. - [Read Microinjecting C.elegans Worms Protocol]

Setting Up Arabidopsis Crosses Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/28/pdb.prot4623

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Arabidopsis naturally self-pollinates, the generation of cross-progeny requires some intervention by the investigator. This protocol describes the generation and collection of seeds by crossing suitable Arabidopsis parent plants. - [Read Setting Up Arabidopsis Crosses Protocol]

Setting Up Microdrop Cultures Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4353

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Protocol describes how to set up microdrop cultures to produce embryos which can then be used for making chimeras. The microdrop culture should be set up several hours to 1 day before the experiment to permit temperature and gas equilibration. - [Read Setting Up Microdrop Cultures Protocol]