senescent Protocols

Category: Immunology: Immunofluorescence Protocols: Immunofluorescence Staining Protocols

Protocol for immunostaining of DDR proteins in senescent cells. Detailed methodology to study the activation of a number of DNA damage response factors in senescent human fibroblasts. - [Read Immunostaining of DDR Proteins in Senescent Cells Protocol]

Category: RNAi Technology Protocols: RNAi Mammalian Cells Protocols

Protocol for oligofection of small interfering RNAs in senescent human fibroblasts. This protocol allows the transfection of human senescent cells (regardless of the cause of cellular senescence) with an efficiency of around 70%. - [Read Oligofection of Small Interfering RNAs in Senescent Human Fibroblasts Protocol]