semi Protocols

Search results for: semi

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

A semi-permanent mounting medium for immunofluorescence microscopy - http://www.hei.org/research/aemi/moviol.htm

Category: Molecular Imaging: Light Microscopy

A semi-permanent mounting medium for immunofluorescence microscopy. House Ear Institute - [Read A semi-permanent mounting medium for immunofluorescence microscopy]

Bisulfite-PCR for Restriction Analysis and/or Sequencing Protocol - http://www.mdanderson.org/departments/methylation/display.cfm?id=8E0AAAAC-FA42-486C-A93F60CD6E1C7E51&method=displayFull&pn=A3F15D82-C9B4-41CD-BA4BE767E50A73D2

Category: PCR Protocols: Bisulfite Conversion Based PCR Protocols

Protocol for bisulfite-PCR for restriction analysis and/or sequencing. Bisulfite-PCR followed by restriction is a rapid and semi-quantitative method of analyzing DNA methylation. The PCR products are also suitable for either direct sequencing or cloning and sequencing. The most important step here is primer selection. - [Read Bisulfite-PCR for Restriction Analysis and/or Sequencing Protocol]

DNA Extraction from Agarose Gels Protocol - http://www.methodbook.net/dna/gelextrc.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

DNA Extraction from Agarose Gels Protocol. The page includes cutting out the DNA band from the gel, and describes three methods including 1) Spin-columns (Nucleic acid purification columns), 2) using Dialysis tubing (semi-permeable membrane, Visking tubing), and the 3) Paper strip method.Matt Lewis, Department of Pathology, University of Liverpool. - [Read DNA Extraction from Agarose Gels Protocol]

Drosophila Growth Conditions Protocol - http://flyrnai.org/RNAi_FAQ_dros_cc.html#III.

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila growth conditions. Includes: MAINTENANCE; Semi-adherent cell lines; Adherent cell lines; SL2, S2C, S2*; DL2, DL1, S2R+, Kc167, Clone 8, S3; BG2. - [Read Drosophila Growth Conditions Protocol]

Drosophila Semi-defined Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/germanfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila semi-defined medium. - [Read Drosophila Semi-defined Medium Protocol]

Enzyme Immunoaffinity Chromatographyâ€”A Rapid Semi-quantitative Method - http://www.rsc.org/delivery/_ArticleLinking/DisplayArticleForFree.cfm?doi=a607578i

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Enzyme Immunoaffinity Chromatographyâ€”A Rapid Semi-quantitative Method. - [Read Enzyme Immunoaffinity Chromatographyâ€”A Rapid Semi-quantitative Method]

Immunoblotting: Semi-Dry Electrophoretic Transfer of Proteins from Gels to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4301

Category: Western Blot Protocols: General Western Blot Methods Protocols

The transfer of proteins from a Tris/glycine SDS-polyacrylamide gel to a membrane using a semi-dry method is achieved by placing the gel next to a piece of nitrocellulose filter. This sandwich is placed directly between two plate electrodes, and the proteins are then transferred from the gel onto the filter. - [Read Immunoblotting: Semi-Dry Electrophoretic Transfer of Proteins from Gels to Membranes Protocol]

Light Microscopy Techniques - http://www.hei.org/research/aemi/clm.htm

Category: Molecular Imaging: Light Microscopy

Ahmanson Center for Advanced Electron Microscopy and Imaging. Use of Semi-Thin Cryosections for Light Microscopy, Immunolabeling of Cryosections on Glass Slides, Problems with Autofluorescence. House Ear Institute - [Read Light Microscopy Techniques]

Semi-Quantitative Measurement of Proteins by Dot Blotting - http://www.cbrinstitute.org/labs/springer/protocols/jun_dotblot.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Semi-Quantitative Measurement of Proteins by Dot Blotting. Jun Takagi Springer Lab. Includes protein quantitation, nitrocellulose, antibody, chemiluminescence, peroxidase.

- [Read Semi-Quantitative Measurement of Proteins by Dot Blotting]