seeds Protocols

Search results for: seeds

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Clearing Arabidopsis Ovule Materials with the Hoyer's Solution Protocol - http://www.hybtech.org/Liu/Hoyer_clearing.htm

Protocol can be used for clearing intact seeds of arabidopsis, which can then be observed under Normarski optics. This is an efficient way to analyse embryo and endosperm development without sectioning. - [Read Clearing Arabidopsis Ovule Materials with the Hoyer's Solution Protocol]

Cultivation of Arabidopsis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/27/pdb.ip22

Category: Plant Biology Protocols

In most natural habitats, Arabidopsis is a winter annual: Its seeds germinate in the fall, the young plants survive the winter, floral meristems emerge in the spring, and only the seeds survive the summer months. Most common laboratory varieties of Arabidopsis flower within 4 weeks of germination, and seeds can be collected after an additional 4-6 weeks. - [Read Cultivation of Arabidopsis Protocol]

Extraction and Solubilization of Total Protein from Plant Seeds Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4225

Category: Plant Biology Protocols: Plant Proteins, Peptides and Antigens

Protocol on the extraction and solubilization of total protein from plant seeds. - [Read Extraction and Solubilization of Total Protein from Plant Seeds Protocol]

In Vitro Growth of Seedlings - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/PlantWork/invitroGrowthSeedlings.html

Category: Plant Biology Protocols

Protocol of in vitro growth of seedlings includes: sterilisation of seeds; plating and growth. - [Read In Vitro Growth of Seedlings]

Making Microtubule Seeds Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/mtseeds.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for making microtubule seeds. - [Read Making Microtubule Seeds Protocol]

Setting Up Arabidopsis Crosses Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/28/pdb.prot4623

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Arabidopsis naturally self-pollinates, the generation of cross-progeny requires some intervention by the investigator. This protocol describes the generation and collection of seeds by crossing suitable Arabidopsis parent plants. - [Read Setting Up Arabidopsis Crosses Protocol]

Articles (1-1 of 1)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.