seeded Protocols

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Passage Procedure for RAW 264.7 Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000159.pdf?pid=PP00000159

Category: Cell Culture Protocols: Cell Lines Protocols

RAW 264.7 cells are a macrophage-like, Abelson leukemia virus transformed cell line derived from BALB/c mice. For routine maintenance in culture (passage), cells are seeded at a confluence of approximately 10% (1 x 106 and 3 x 106 cells in 100-mm and 150-mm plates, respectively) and grown to a confluence of approximately 80%. This procedure requires the cells to be split every two days. - [Read Passage Procedure for RAW 264.7 Cells]

Preparation of Seeded NGM Plates For Worm Food Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1558

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Protocol describes how to seed plates with E. coli OP50 for C. elegans cultures. Includes: Bacterial Stock Plate Preparation; Bacterial Broth Preparation; Seeding Bacteria Plates for Culturing Worms. - [Read Preparation of Seeded NGM Plates For Worm Food Protocol]

Seeded films (Vorm-Roepstorff) MALDI Protocol - http://prowl.rockefeller.edu/methods/seeded.htm

Category: Mass Spectrometry Protocols

This sample preparation method is a direct replacement for the dried droplet technique. It produces more uniform samples than the dried-droplet technique and the crystals are better adhered to the sample stageâ€™s surface. MALDI Sample preparation. PROWL - [Read Seeded films (Vorm-Roepstorff) MALDI Protocol]

Treatment of Cells with 5-aza-dC. protocol PDF - http://www.shmu.edu.cn/courses/2005aut/upload/20051116/Hongmei%20Xu%202003%20CANCER%20RESEARCH%20%20Aberrant%20Methylation%20and%20Silencing%20of%20ARHI,%20an%20Imprinted%20Tumor%20Suppressor%20Gene%20in%20which%20the%20Function%20Is%20Lost%20in%20Breast%

Category: Epigenetics and Methylation Protocols: 5-Aza-dC Treatment AZA Procedure

"Cells were seeded at a density of 1X106 cells/100-mm dish with 10% FBS and allowed to attach over a 24-h period. 5-Aza-dC (Sigma) was then added to a final concentration of 0.2â€“1 M, and the cells were allowed to grow for 5 days. The medium with or w - [Read Treatment of Cells with 5-aza-dC. protocol PDF]