screens Protocols

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Benton Davis Blots for cDNA Libraries - http://omrf.ouhsc.edu/~frank/BENTNDAV.html

Category: RNA Protocols: cDNA Libraries Library

Includes procedures for cDNA screens using secondary and tertiary screening. Dr. Bart Frank Lab. - [Read Benton Davis Blots for cDNA Libraries]

Gateway-Compatible Yeast One-Hybrid Screens Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/28/pdb.prot4590

Category: Yeast Protocols: Yeast Nucleic Acid Protocols

Protocol for gateway-compatible yeast one-hybrid screens. - [Read Gateway-Compatible Yeast One-Hybrid Screens Protocol]

Microspheres for High-Throughput Screening Assays - http://www.pall.com/pdf/oep_hts_brochure.pdf

Category: Microsphere Analysis Protocols

The role of microspheres in these screens is similar to their traditional role in immunoassays, namely as a solid phase to either enhance detection, separation, or both. The predominance of radioactive assays in high-throughput screening, along with the desire to find alternative means of detection, have led to research on substituting alternative fluorescent technologies. - [Read Microspheres for High-Throughput Screening Assays]

Monitoring Protein Phosphorylation for the Ligand Screens PPT - http://www.signaling-gateway.org/aboutus/supplement/AM2003/10a_Mumby.ppt

Category: Protein Protocols: Protein Phosphorylation Protocols

A powerpoint slide demonstrating a method for Monitoring Protein Phosphorylation for Ligand Screens. Mumby. signaling-gateway.org - [Read Monitoring Protein Phosphorylation for the Ligand Screens PPT]

PCR Screens of ES Cell Clones - http://www.fhcrc.org/science/labs/soriano/protocols/pcres.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Identification of targeted ES cell clones by PCR. Fred Hutchinson Cancer Research Center. - [Read PCR Screens of ES Cell Clones]

PCR Screens of ES Cell Clones - http://www.fhcrc.org/science/labs/soriano/protocols/pcres.html

Category: PCR Protocols

Identification of targeted ES cell clones by PCR. Fred Hutchinson Cancer Research Center. - [Read PCR Screens of ES Cell Clones]

reparation of Copy Standards for Southern Blot Copy Number Determination - http://www.med.umich.edu/tamc/spike.html

Category: Transgenic Mouse Protocols: Mouse Genotyping Protocols

PCR screens must be designed to detect transgene DNA at the single copy level.Copy standards are prepared by mixing non-transgenic tail DNA with a known amount of transgene DNA to produce transgene copy standards. University of Michigan Transgenic Animal - [Read reparation of Copy Standards for Southern Blot Copy Number Determination]

RNAi Screens in C. elegans Protocol - http://www.natureprotocols.com/2006/07/19/rnai_screens_in_c_elegans_in_a.php

Category: RNAi Technology Protocols: RNAi C. elegans Protocols

Protocol for RNAi screens in C. elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions. The procedure allows thousands of RNAi feeding experiments to be performed per investigator per day. - [Read RNAi Screens in C. elegans Protocol]