Protocol for microingecting C.elegans worms. Includes: Making the DNA solution; Setting up the scope, loading the needle, mounting, and breaking the needle; Mounting worms on an injection pad; Injecting. - [Read Microinjecting C.elegans Worms Protocol]
Protocol for nematode dye filling. Includes: Thumb plate; Microfuge tube; 15 ml tube; Viewing: Big Scope and Dissecting Scope. - [Read Nematode Dye Filling Protocol]
This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.