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Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates - http://www.glycotech.com/protocols/Proto9.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Protocol detects specific cell adhesion to glycolipids resolved on TLC plates. Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates. Ronald L. Schnaar~Professor, Johns Hopkins University Medical School, Baltimore, Maryland. Glycotech. - [Read Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates]

DNA digestion protocol & hints PDF - http://www.crypto.wustl.edu/Protocols%20PDFs/RE%20digests%20(HL).pdf

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

DNA digestion protocol & hints. Procedure and tips for restriction digestion. Doering laboratory, Department Molecular Microbiology, Washington University Medical School. - [Read DNA digestion protocol & hints PDF]

DNA Extraction From Blood Leukocytes - http://geneticslab.topcities.com/DNAextraction.htm

Category: DNA Protocols: DNA Extraction Protocols

DNA extracted from peripheral blood leucocytes using 3mls of whole blood. Modification of a salting out procedure as described by Miller et al., (1988). The DNA Laboratory, Medical School, Malta. - [Read DNA Extraction From Blood Leukocytes]

DNA Extraction from Cheek Cells - http://geneticslab.topcities.com/cheekcells.htm

Category: DNA Protocols: DNA Extraction Protocols

Cheek cells obtained by rinsing the mouth commercial mouth wash solution. Mouth wash is then discarded into a sterile conical tube and sent to the lab. Based on Salting out procedure. DNA Laboratory, Medical School, Malta. - [Read DNA Extraction from Cheek Cells]

dsRNA Synthesis Protocol - http://flyrnai.org/RNAi_FAQ_dsrna_syn.html

Category: siRNA and RNAi Protocols

dsRNA From Clones, Primer Designed dsRNA. Drosophila RNAi Screening Center at Harvard Medical School - [Read dsRNA Synthesis Protocol]

ES cell culture - http://saturn.med.nyu.edu/research/mp/ronlab/protocols/ES_cell_culture.html

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

Heather P. Harding, NYU School of Medicine - [Read ES cell culture]

ES Cell Culture and Manipulation - http://web.archive.org/web/20040301061220/http://dicty.cmb.nwu.edu/Chis_lab/Lab+Manual/es_cell_culture_and_manipulation.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Very detailed protocol. ES Cell Culture and Manipulation. Chisholm Lab, Northwestern University Medical School. - [Read ES Cell Culture and Manipulation]

FACS Procedures for Apoptosis Detection TUNEL - http://biochem.dental.upenn.edu/~sdmfacs/apopproc.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

FACS Procedures for Apoptosis Detection with TUNEL. U. Pennsylvania Dental School. - [Read FACS Procedures for Apoptosis Detection TUNEL]

Formaldehyde Treatment of Tissue Culture Hoods - http://www.nottingham.ac.uk/~mbzspd/methods/Formaldehyde_Treatme.html

Category: General Research Protocols and Methods: Sterile Technique

Formaldehyde sterilization of tissue culture hoods. A bit toxic however effective. Dr.Dawson, Department Biochemistry, Univ. Nottingham Medical School. - [Read Formaldehyde Treatment of Tissue Culture Hoods]

FPLC Protocol - http://users.path.ox.ac.uk/~ciu/Methods/Protein/fplc.html

Category: Chromatography Protocols

FPLC Protocol. The FPLC consists of a pump and a column which will withstand high pressure so separations can be carried out relatively quickly. For a detailed description there is a FPLC system handbook which is particularly useful for trouble shooting. For use of individual columns follow the "instructions" (in the green folder) which accompany each one. Sir William Dunn School of Pathology, Oxford University. - [Read FPLC Protocol]

Isolation of DNA from Strawberries and Raspberries - http://www.science-projects.com/onionDNA.htm

Category: DNA Protocols: DNA Extraction Protocols

Also works for onions. Duration ~40 minutes. DNA Isolation with very easily accessible household solutions. Great for school classes. science-projects.com - [Read Isolation of DNA from Strawberries and Raspberries]

List of standard EM Center procedures/protocols - http://anatomy.iupui.edu/emcenter/protocols.html

Category: Molecular Imaging: Electron Microscopy Protocols

Protocol for sectioning is on a separate page. 2. Standard Procedure To Section for Transmission Electron Microscopy, EM Center, Indiana University School ... List of standard EM Center procedures/protocols - [Read List of standard EM Center procedures/protocols]

Lowry Protein Assay Protocol - http://ylwang.umassmed.edu/protocol/pa/lowry.htm

Category: Protein Protocols: Lowry Protein Assay

Lowry Protein Assay Protocol. Yu-li Wang Lab, Univ. Massachusetts Medical School. Detailed protocol including materials. - [Read Lowry Protein Assay Protocol]

SECTIONING RESIN EMBEDDED TISSUE - http://www.nottingham.ac.uk/pathology/protocols/resinsect.html

Category: Histology Protocols: Tissue Sample Sectioning

SECTIONING RESIN EMBEDDED TISSUE. MAKING LATTA-HARTMANN KNIVES and Sectioning Protocols for Histology. University of Nottingham Medical School Division of Histopathology. James Lowe. - [Read SECTIONING RESIN EMBEDDED TISSUE]