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Detection of Mutations by Single-strand Conformational Polymorphism Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3815

Category: Genetics and Genomics: Cancer Genetics Protocols: Single Strand Conformational Polymorphism(SSCP) Protocols

Single-strand conformational polymorphism (SSCP), one of several methods used to scan segments of DNA for mutations, exploits the electrophoretic differences in mobilities between single-stranded mutant and wild-type DNAs. - [Read Detection of Mutations by Single-strand Conformational Polymorphism Protocol]

Spotted Microarray Hybridization and Scan Protocol - http://www.genome.wisc.edu/resources/protocols/spottedarrayhybridization.htm

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol for spotted microarray hybridization and scan. Includes: Setup Hybridization; Wash Slides; Scan Slides. - [Read Spotted Microarray Hybridization and Scan Protocol]

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Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.